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首页> 外文期刊>BMC Microbiology >Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor model
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Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor model

机译：使用单个反应器模型评估格氏乳杆菌K7和双歧杆菌从胃到肠的传递

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Background Probiotic bacteria are thought to play an important role in the digestive system and therefore have to survive the passage from stomach to intestines. Recently, a novel approach to simulate the passage from stomach to intestines in a single bioreactor was developed. The advantage of this automated one reactor system was the ability to test the influence of acid, bile salts and pancreatin. Lactobacillus gasseri K7 is a strain isolated from infant faeces with properties making the strain interesting for cheese production. In this study, a single reactor system was used to evaluate the survival of L. gasseri K7 and selected bifidobacteria from our collection through the stomach-intestine passage. Results Initial screening for acid resistance in acidified culture media showed a low tolerance of Bifidobacterium dentium for this condition indicating low survival in the passage. Similar results were achieved with B. longum subsp. infantis whereas B. animalis subsp. lactis had a high survival. These initial results were confirmed in the bioreactor model of the stomach-intestine passage. B. animalis subsp. lactis had the highest survival rate (10%) attaining approximately 5 × 106 cfu ml-1 compared to the other tested bifidobacteria strains which were reduced by a factor of up to 106. Lactobacillus gasseri K7 was less resistant than B. animalis subsp. lactis but survived at cell concentrations approximately 1000 times higher than other bifidobacteria. Conclusion In this study, we were able to show that L. gasseri K7 had a high survival rate in the stomach-intestine passage. By comparing the results with a previous study in piglets we could confirm the reliability of our simulation. Of the tested bifidobacteria strains, only B. animalis subsp. lactis showed acceptable survival for a successful passage in the simulation system.
机译：背景技术益生菌被认为在消化系统中起着重要作用，因此必须在从胃到肠的通道中生存。最近，开发了在单个生物反应器中模拟从胃到肠的通过的新颖方法。这种自动化的单反应器系统的优势在于能够测试酸，胆汁盐和胰酶的影响。 Gasseri gasseri K7乳杆菌是从婴儿粪便中分离的菌株，其特性使该菌株对于奶酪生产很有趣。在这项研究中，使用单个反应器系统评估胃泌乳道肠杆菌L. gasseri K7和选自我们收集的双歧杆菌的存活率。结果在酸化培养基中对耐酸性的初步筛选显示，在这种情况下，牙本质双歧杆菌耐受性低，表明传代存活率较低。长双歧杆菌亚种获得了相似的结果。婴儿，而动物分枝杆菌亚种。乳酸有很高的存活率。这些初步结果在胃-肠通道的生物反应器模型中得到证实。 B.动物亚种与其他测试的双歧杆菌菌株相比，乳酸菌具有最高的存活率（10％），达到约5×10 6 cfu ml -1 10 6 。加氏乳杆菌K7的抗药性低于动物双歧杆菌亚种。乳酸菌，但在比其他双歧杆菌高约1000倍的细胞浓度下存活。结论在这项研究中，我们能够证明加氏乳杆菌K7在胃-肠通道中具有较高的存活率。通过将结果与先前在仔猪中的研究进行比较，我们可以证实我们模拟的可靠性。在测试的双歧杆菌菌株中，仅动物双歧杆菌亚种。乳酸菌在模拟系统中成功传代显示出可接受的存活率。

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