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首页> 外文期刊>BMC Microbiology >Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by Paenibacillus elgii
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Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by Paenibacillus elgii

机译：埃尔氏帕尼杆菌产生的环状脂肽抗生素pelgipeptin生物合成中涉及基因簇的鉴定和功能分析

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Background Pelgipeptin, a potent antibacterial and antifungal agent, is a non-ribosomally synthesised lipopeptide antibiotic. This compound consists of a β-hydroxy fatty acid and nine amino acids. To date, there is no information about its biosynthetic pathway. Results A potential pelgipeptin synthetase gene cluster (plp) was identified from Paenibacillus elgii B69 through genome analysis. The gene cluster spans 40.8?kb with eight open reading frames. Among the genes in this cluster, three large genes, plpD, plpE, and plpF, were shown to encode non-ribosomal peptide synthetases (NRPSs), with one, seven, and one module(s), respectively. Bioinformatic analysis of the substrate specificity of all nine adenylation domains indicated that the sequence of the NRPS modules is well collinear with the order of amino acids in pelgipeptin. Additional biochemical analysis of four recombinant adenylation domains (PlpD A1, PlpE A1, PlpE A3, and PlpF A1) provided further evidence that the plp gene cluster involved in pelgipeptin biosynthesis. Conclusions In this study, a gene cluster (plp) responsible for the biosynthesis of pelgipeptin was identified from the genome sequence of Paenibacillus elgii B69. The identification of the plp gene cluster provides an opportunity to develop novel lipopeptide antibiotics by genetic engineering.
机译：背景技术Pelgipeptin是一种有效的抗菌和抗真菌剂，是一种非核糖体合成的脂肽抗生素。该化合物由β-羟基脂肪酸和九个氨基酸组成。迄今为止，还没有关于其生物合成途径的信息。结果通过基因组分析，从埃尔氏Paenibacillus elgii B69中鉴定出潜在的pelpipeptin合成酶基因簇（plp）。基因簇跨越40.8kb，具有八个开放阅读框。在该簇中的基因中，显示三个大基因plpD，plpE和plpF编码具有一个，七个和一个模块的非核糖体肽合成酶（NRPS）。对所有九个腺苷酸化结构域的底物特异性的生物信息学分析表明，NRPS模块的序列与聚肽素中的氨基酸顺序很好地共线。四个重组腺苷酸化域（PlpD A1，PlpE A1，PlpE A3和PlpF A1）的其他生化分析提供了进一步的证据，表明plp基因簇参与了pelgipeptin生物合成。结论在这项研究中，从帕氏芽孢杆菌B69的基因组序列中鉴定了负责pelgipeptin生物合成的基因簇（plp）。 plp基因簇的鉴定为通过基因工程开发新型脂肽抗生素提供了机会。

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