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首页> 外文期刊>BMC Microbiology >Proteomic comparison of the cytosolic proteins of three Bifidobacterium longum human isolates and B. longum NCC2705
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Proteomic comparison of the cytosolic proteins of three Bifidobacterium longum human isolates and B. longum NCC2705

机译：三种长双歧杆菌人分离株和长双歧杆菌NCC2705胞质蛋白的蛋白质组学比较

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Background Bifidobacteria are natural inhabitants of the human gastrointestinal tract. In full-term newborns, these bacteria are acquired from the mother during delivery and rapidly become the predominant organisms in the intestinal microbiota. Bifidobacteria contribute to the establishment of healthy intestinal ecology and can confer health benefits to their host. Consequently, there is growing interest in bifidobacteria, and various strains are currently used as probiotic components in functional food products. However, the probiotic effects have been reported to be strain-specific. There is thus a need to better understand the determinants of the observed benefits provided by these probiotics. Our objective was to compare three human B. longum isolates with the sequenced model strain B. longum NCC2705 at the chromosome and proteome levels. Results Pulsed field electrophoresis genotyping revealed genetic heterogeneity with low intraspecies strain relatedness among the four strains tested. Using two-dimensional gel electrophoresis, we analyzed qualitative differences in the cytosolic protein patterns. There were 45 spots that were present in some strains and absent in others. Spots were excised from the gels and subjected to peptide mass fingerprint analysis for identification. The 45 spots represented 37 proteins, most of which were involved in carbohydrate metabolism and cell wall or cell membrane synthesis. Notably, the protein patterns were correlated with differences in cell membrane properties like surface hydrophobicity and cell agglutination. Conclusion These results showed that proteomic analysis can be valuable for investigating differences in bifidobacterial species and may provide a better understanding of the diversity of bifidobacteria and their potential use as probiotics.
机译：背景双歧杆菌是人类胃肠道的自然居民。在足月新生儿中，这些细菌是在分娩时从母亲那里获得的，并迅速成为肠道菌群中的主要生物。双歧杆菌有助于建立健康的肠道生态，并可以为其宿主带来健康益处。因此，人们对双歧杆菌的兴趣日益增长，目前各种菌株被用作功能性食品中的益生菌成分。然而，据报道益生菌作用是菌株特异性的。因此，需要更好地理解这些益生菌提供的观察到的益处的决定因素。我们的目标是在染色体和蛋白质组水平上，将三种人类长双歧杆菌菌株与测序的长双歧杆菌NCC2705进行比较。结果脉冲场电泳基因分型表明，在所测试的四种菌株中，遗传异质性较低，种内菌株相关性较低。使用二维凝胶电泳，我们分析了胞质蛋白模式的质性差异。在某些菌株中存在45个斑点，而在其他菌株中则不存在。从凝胶上切下斑点，并进行肽质量指纹分析以鉴定。 45个斑点代表37种蛋白质，其中大多数参与碳水化合物的代谢以及细胞壁或细胞膜的合成。值得注意的是，蛋白质模式与细胞膜特性（例如表面疏水性和细胞凝集）的差异相关。结论这些结果表明，蛋白质组学分析对于研究双歧杆菌物种的差异可能具有重要价值，并且可以更好地了解双歧杆菌的多样性及其作为益生菌的潜在用途。

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