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首页> 外文期刊>BMC Molecular Biology >Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma
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Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma

机译：氧化石墨烯下调胶质母细胞瘤中氧化磷酸化复合物的基因

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摘要

Recently different forms of nanographene were proposed as the material with high anticancer potential. However, the mechanism of the suppressive activity of the graphene on cancer development remains unclear. We examined the effect of oxygenated, reduced and pristine graphene on the gene expression in glioblastoma U87 cell line. Conducting microarrays and RT-qPCR analysis we explored that graphene oxide (rather than reduced graphene oxide and pristine graphene) down-regulates the mRNA expression of mitochondrial oxidative phosphorylation (OXPHOS) nuclear genes of complexes I, III, IV and V. The presented results provide first evidence for the hypothesis that the suppressed growth of GBM can be the consequence of down-regulation of OXPHOS protein expression and decreased ATP level. We suggest that changes in the expression of OXPHOS genes identified in our study may mediate the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells. However, further investigations with different cell lines, regarding expression, regulation and activity of OXPHOS genes identified in our study is necessary to elucidate the mechanism mediating the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells.
机译：最近，提出了不同形式的纳米石墨烯作为具有高抗癌潜力的材料。然而，石墨烯对癌症发展的抑制活性的机制仍不清楚。我们检查了氧化的，还原的和原始的石墨烯对胶质母细胞瘤U87细胞系中基因表达的影响。进行芯片和RT-qPCR分析，我们发现氧化石墨烯（而不是还原的氧化石墨烯和原始石墨烯）下调复合物I，III，IV和V的线粒体氧化磷酸化（OXPHOS）核基因的mRNA表达。为以下假设提供了第一个证据：GBM的抑制生长可能是OXPHOS蛋白表达下调和ATP水平降低的结果。我们建议在我们的研究中确定的OXPHOS基因表达的变化可能介导胶质母细胞瘤细胞中氧化石墨烯的抗增殖和抗迁移作用。然而，在我们的研究中鉴定出的有关OXPHOS基因的表达，调控和活性的不同细胞系的进一步研究，对于阐明介导氧化石墨烯在胶质母细胞瘤细胞中的抗增殖和抗迁移作用的机制是必要的。

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