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首页> 外文期刊>British Biotechnology Journal >Development of an Efficient Plant RegenerationSystem of Field Mustard (Brassica campestris)
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Development of an Efficient Plant RegenerationSystem of Field Mustard (Brassica campestris)

机译：野芥菜（Brassica campestris）高效植物更新系统的开发

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Aims: The present study was conducted with a view to develop an efficient protocol for high frequency plant regeneration of Brassica campestris for further crop improvement program by biotechnological manipulation and to optimize this system for regeneration of a number of B. campestris genotypes.Study Design: Completely Randomized Design.Place and Duration of Study: This experiment was carried out in the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Bangladesh during the period of July 2013 to June 2014.Methodology: Cotyledon and hypocotyl explants of B. campestris cv. BARI sarisha-12 were cultured on MS medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA) for callus initiation and shoot regeneration. Later on subsequent subculturing is done for shoot elongation and multiplication. MS medium supplemented with various concentrations of NAA were used for root formation.Results: From a total of 15 different combinations of BAP and NAA tested, the combination of 1.0 mg L-1 BAP and 0.5 mg L-1 NAA gave the highest frequency of callus initiation (94.44%) as well as shoot regeneration (63.89%) in case of cotyledon explants where as hypocotyl explants showed 47.62% callus initiation and 19.04% shoot regeneration frequency. Four days old cotyledon explants showed the highest shoot regeneration frequency (72.22%) and higher number of shoots per explant (3.94) than those from older seedling. The shoot regeneration frequency markedly enhanced to 83.33% by the addition of 2.0 mg L-1 AgNO3 to the MS medium supplemented with 1.0 mg L-1 BAP, 0.5 mg L-1 NAA and this combination also showed the maximum number of shoots per explant (6.86). Shoot regeneration potentiality of five B. campestris genotypes were investigated and indicated that this system would be widely applicable to all the genotypes. The regenerated shoots were easily rooted on MS medium supplemented with 0.2 mg L-1 NAA and the whole plants were transferred to pot soils and grown to maturity.Conclusion: MS medium supplemented with 1.0 mg L-1 BAP, 0.5 mg L-1 NAA and 2.0 mg L-1 AgNO3 is more efficient for multiple shoot regeneration by using cotyledon explants and it may be utilized for In vitro improvement program of B. campestris.
机译：目的：进行本研究的目的是开发一种有效的甘蓝型油菜高频植物再生方案，以通过生物技术操作进一步改良作物，并优化该系统以再生多种油菜型油菜基因型。研究设计：完全随机设计研究地点和持续时间：该实验于2013年7月至2014年6月在孟加拉国锡尔赫特农业大学遗传与植物育种系遗传工程实验室进行。方法：子叶和下胚轴外植体B. campestris简历BARI sarisha-12在补充了不同浓度的6-苄基氨基嘌呤（BAP）和α-萘乙酸（NAA）的MS培养基上培养，用于愈伤组织引发和芽再生。稍后进行继代培养，以进行芽伸长和繁殖。结果：从总共测试的15种BAP和NAA组合中，1.0 mg L-1 BAP和0.5 mg L-1 NAA的组合出现频率最高。在子叶外植体的情况下，愈伤组织起始（94.44 ％）和枝条再生（63.89 ％），其中下胚轴外植体显示47.62 ％的愈伤组织起始和19.04 ％枝条再生频率。四天大的子叶外植体显示出最高的芽再生频率（72.22％），每株外植体的芽数（3.94）高于老苗。通过向添加了1.0 mg L-1 BAP，0.5 mg L-1 NAA的MS培养基中添加2.0 mg L-1 AgNO3，可将芽的再生频率显着提高至83.33％。外植体（6.86）。研究了5个营地油菜B. campestris基因型的芽再生潜力，并表明该系统将广泛适用于所有基因型。再生的芽很容易生根于补充有0.2 mg L-1 NAA的MS培养基上，并将整株植物转移到盆栽土壤中生长至成熟。结论：补充有1.0 mg L-1 BAP，0.5 mg L-1 NAA的MS培养基通过使用子叶外植体，2.0 mg L-1 AgNO3可以更有效地进行多芽再生，可用于野菜双歧杆菌的体外改良计划。

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