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首页> 外文期刊>British Biotechnology Journal >Isolation and 16S rRNA-Based Identification ofBenomyl-Degrading Bacteria
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Isolation and 16S rRNA-Based Identification ofBenomyl-Degrading Bacteria

机译：苯菌灵降解菌的分离和基于16S rRNA的鉴定

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Laboratory experiments were conducted to isolate and identify Benlate- (Benomyl) degrading microorganisms from two soil types collected from different locations in Khartoum State, Sudan. Benomyl degradation was studied at two temperatures (28 and 40oC) in soil treated with three Benomyl concentrations (0.032, 3.2 and 8.0mg Benomyl/g soil) and incubated for 360 days. Potential degraders were also tested in mineral salt liquid medium using Benomyl as a sole carbon source. Degradation percentages were then determined and the most efficient Benomyl degraders were identified by amplification with 16S rRNA gene, sequencing and alignment with deposited sequences in the international gene bank. A total of 64 isolates were recovered from the two soil types, with 59 (92.2%) isolates recovered from the clay soil. Thirty four isolates were recovered from clay soil treated with 8.0mg Benomyl/g soil and incubated at 28oC. The most efficient Benomyl degraders, with degradation percentages in the range of 44-59, were identified as Pseudomonas stutzeri (Two different isolates), Pseudomonas putida, Acinetobacter johnsonii, Brevibacillus invocatus, Bacillus clausii, Lysinibacillus sp. and Agrobacterium radiobacter.
机译：进行了实验室实验，以从苏丹喀土穆州不同地点收集的两种土壤中分离和鉴定降解Benlate-（苯菌灵）的微生物。在三种浓度的苯菌灵（0.032、3.2和8.0mg苯菌灵/ g土壤）处理的土壤中，在两个温度（28和40oC）下研究了苯菌灵的降解，并孵育了360天。还使用苯菌灵作为唯一碳源，在矿物盐液体培养基中测试了潜在的降解剂。然后确定降解百分比，并通过用16S rRNA基因扩增，测序以及与国际基因库中存放的序列进行比对，确定最有效的苯菌灵降解剂。从这两种土壤类型中总共回收了64种分离株，其中从黏土中回收了59种（92.2％）分离株。从用8.0mg苯菌灵/ g土壤处理过的黏土中分离出34株分离物，并在28oC下孵育。降解百分比在44-59之间的最有效的苯菌灵降解剂被鉴定为斯图氏假单胞菌（两种不同的分离株），恶臭假单胞菌，不动杆菌，约翰内氏短杆菌，invocatus，克劳氏芽孢杆菌，Lysinibacillus sp.。和农杆菌

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