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Rapid micropropagation of Cucumis sativusvar. Dastgerdi (Iranian cultivar) by Node CultureTechnique

机译:黄瓜的快速微繁殖。 Node CultureTechnique撰写的Dastgerdi(伊朗品种)

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Tissue culture can be used in developing an efficient method for production of valuable and enduring indigenous cultivars. A short-term protocol for plantlet proliferation by using nodal segments was developed for Cucumis sativus, local cultivar from Isfahan; known as Dastgerdi as a tolerant cultivar to the root-knot nematode. Nodal explants were cultured on MS medium containing various concentrations of KIN (0, 0.5, 1 and 1.5mg.l-1) in combination with IBA (0, 0.025 and 0.5mg.l-1). The best response for proliferation rate (100%), mean number of nodes per plantlet (6.01) and percentage of rooted In vitro propagated shoots were observed on medium supplemented by KIN (1.5mg.l-1) after about 3-4 weeks. The maximum length (5.43cm) of micro-shoots obtained on MS medium with KIN (1mg.l-l) + IBA (0.025mg.l-1).
机译:组织培养可用于开发生产有价值和持久的本土品种的有效方法。针对来自伊斯法罕(Isfahan)的本地栽培种黄瓜(Cucumis sativus),开发了一种利用节段的短期繁殖方案。被称为Dastgerdi,是对根结线虫的耐受性品种。在含有各种浓度的KIN(0、0.5、1和1.5mg.1-1)和IBA(0、0.025和0.5mg.1-1)的MS培养基上培养结节外植体。大约3-4周后,在补充KIN(1.5mg.l-1)的培养基上观察到最佳的增殖率(100%),每株小结平均节数(6.01)和生根的体外繁殖枝的最佳响应。在具有KIN(1mg.1-1)+ IBA(0.025mg.1-1)的MS介质上获得的微芽的最大长度(5.43cm)。

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