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首页> 外文期刊>British Biotechnology Journal >Study on Flowering Time SSR Marker RM 248 inTraditional Rice Accessions in Sri Lanka
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Study on Flowering Time SSR Marker RM 248 inTraditional Rice Accessions in Sri Lanka

机译：斯里兰卡传统水稻品种开花时间SSR标记RM 248的研究

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Aims: To study the diversity of flowering time locus RM248 in some traditional rice cultivars in Sri Lanka and compare it with days to flowering of studied rice cultivars.Study Design: A field experiment was carried out to evaluate the days to flowering of the studied traditional rice cultivars. Rice plants were transplanted according to randomized complete block design with four replications and 20 plants per plot with 15 cm X 20 cm plant spacing. Diversity of flowering time locus RM 248 was analyzed by performing PCR using SSR marker RM 248 with genomic DNA of different eighty-nine rice genotypes. Gel electrophoresis was carried out in vertical gel electrophoresis unit for three hours. Gel plates were Silver stained and banding pattern was scored.Place and Duration of Study: Field data were collected in Sri Lanka. The experiment was carried out during 2011/2012 Maha season and 2012 Yala season at the Faculty of Agriculture, University of Ruhuna, Sri Lanka. In Sri Lanka Yala is referred as dry season while Maha is referred as wet season. Laboratory data were collected at the Laboratory of Genetics, Graduate school of Science and Technology, Kobe University, Japan from October 2012 – December 2012.Methodology: Days-to-flowering of traditional rice cultivars was studied in a field experiment and diversity of flowering time locus, RM 248 was studied by PCR amplification of SSR marker RM 248. SSR marker was amplified using the Gene Amp PCR System 9700 thermocycler. Amplified products were fractionated by electrophoresis through 6% denaturing Polyacrylamide gels and stained using Silver sequence DNA sequencing system staining regents (Promega, USA).Results: Silver stained Polyacrylamide gel showed six different allele types in traditional rice accessions in RM 248 locus.Conclusion: There is a great diversity in the RM248 locus among the traditional rice cultivars in Sri Lanka.
机译：目的：研究斯里兰卡一些传统水稻品种的开花时间位点RM248的多样性，并将其与研究水稻品种的开花天数进行比较。研究设计：进行田间试验，以评价传统水稻品种的开花天数。水稻品种。根据随机完全区组设计，将水稻植株移植四次重复，每块地种植20株植物，株距为15 cm X 20 cm。通过使用SSR标记RM 248与不同八十九个水稻基因型的基因组DNA进行PCR，分析了开花时间基因座RM 248的多样性。在垂直凝胶电泳仪中进行凝胶电泳3小时。凝胶板用银染，并标记带状图。研究的地点和持续时间：在斯里兰卡收集现场数据。该实验在斯里兰卡Ruhuna大学农业学院的2011/2012 Maha季和2012 Yala季进行。在斯里兰卡，Yala被称为旱季，而Maha被称为雨季。 2012年10月至2012年12月在日本神户大学科学技术研究生院遗传学实验室收集了实验室数据。方法：通过田间试验研究了传统水稻品种的开花天数和开花时间的多样性通过对SSR标记RM 248进行PCR扩增研究了RM248。使用Gene Amp PCR System 9700热循环仪对SSR标记进行了扩增。将扩增的产物通过6％变性聚丙烯酰胺凝胶电泳进行分级分离，并使用Silver序列DNA测序系统染色剂（美国Promega）进行染色。结果：银染色的聚丙烯酰胺凝胶在RM 248位点显示传统水稻种中有6种不同的等位基因类型。 ：斯里兰卡的传统水稻品种中，RM248基因座的多样性很大。

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