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首页> 外文期刊>British Biotechnology Journal >Antibacterial and Hemagglutinating Activity of the Fruit Pulp of Trilepisium madagascariense DC
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Antibacterial and Hemagglutinating Activity of the Fruit Pulp of Trilepisium madagascariense DC

机译：马达加斯加特里氏疟原虫果肉浆的抗菌和血凝活性

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Aim: This study aimed at exploring the hemagglutinating and antibacterial activities of crude lectin from the fruit pulp of Trilepisium madagascariense DC. Methods: Crude lectin was obtained by maceration of the fruit and ammonium sulphate precipitation. Hemagglutinating activity assay was carried out by determining the ability of the crude extract to agglutinate red blood cells, serial dilution of the crude lectin was done followed by addition of erythrocytes. The crude lectin was subjected to evaluation for inhibition of bacterial growth by the agar well diffusion method against fourteen human pathogenic gram-positive and gram-negative bacteria. Results: The extract agglutinated trypsinized and glutaraldehyde-fixed rabbit and human erythrocytes with higher specificity for rabbit erythrocytes and better agglutination with trypsinized erythrocytes. Among the various sugar tested, the hemagglutination was best inhibited by galactose. The crude lectin demonstrated mild spectrum antibacterial activity against four pathogenic gram-positive and gram-negative bacteria. The minimum inhibitory concentration ranged from 1.56 mg/ml to 6.25 mg/ml for Streptococcus faecalis, Bacillus anthracis, Staphylococcus aureus and Pseudomonas fluorescens . Conclusion: The study showed that there is presence of hemagglutinins in the extract and the extract possess antibacterial potential against some pathogenic bacteria tested and can be developed as alternative antibacterial drug that could be employed for the treatment of infectious diseases caused by these pathogens.
机译：目的：本研究旨在探讨来自马达加斯加（Trilepisium madagascariense DC）果肉中的粗凝集素的血凝和抗菌活性。方法：通过浸渍水果和硫酸铵沉淀获得粗凝集素。通过确定粗提取物凝集红细胞的能力进行血凝活性测定，对粗凝集素进行连续稀释，然后添加红细胞。通过琼脂井扩散法对粗制的凝集素对14种人类病原性革兰氏阳性和革兰氏阴性细菌的细菌生长抑制进行评估。结果：提取物凝集了胰蛋白酶化和戊二醛固定的兔和人红细胞，对兔红细胞具有更高的特异性，并且与胰蛋白酶化的红细胞具有更好的凝集性。在测试的各种糖中，血凝最好被半乳糖抑制。粗凝集素对四种致病性革兰氏阳性和革兰氏阴性细菌显示出温和的频谱抗菌活性。粪便链球菌，炭疽杆菌，金黄色葡萄球菌和荧光假单胞菌的最低抑菌浓度范围为1.56 mg / ml至6.25 mg / ml。结论：研究表明，提取物中存在血凝素，提取物对某些经测试的病原菌具有抗菌潜力，可作为替代抗菌药物开发，可用于治疗由这些病原体引起的传染病。

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