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首页> 外文期刊>British Biotechnology Journal >DNA Polymorphism and Genetic Variation among Bivoltine Silkworm (Bombyx mori L.) Genotypes Revealed by RAPD Markers
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DNA Polymorphism and Genetic Variation among Bivoltine Silkworm (Bombyx mori L.) Genotypes Revealed by RAPD Markers

机译：RAPD标记揭示的双伏蚕（Bombyx mori L.）基因型之间的DNA多态性和遗传变异

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Background: In breeding programs, the selection of parental lines based on their phenotypic traits along with genetic variation at their DNA level is the better option in directional breeding as specific DNA profiles would be useful in producing reliable estimates of genetic identity and diversity at their genomic levels. In this context, Random amplified polymorphic DNA (RAPD) marker approach was used to ascertain genetic diversity and relationship among 12 bivoltine silkworms ( Bombyx mori L.) genotypes for the identification of diverse genotypes to be used as parents for the improvement of quantitative traits in silkworms. Results: RAPD analysis with 12 silkworm genotypes revealed high polymorphism of 88.88%. Ten RAPD primers utilized in this study generated PCR products in the range of 202-2876 bp across 12 silkworm genotypes. The Polymorphism information content (PIC) ranged from 0.295 to 0.493%. Based on Nei’s similarity coefficients, the genetic distance among the studied silkworm genotypes ranged from 0.93 to 0.99. Of the pairwise combinations, CSR18 and SH6, showed the highest similarity (0.99) whereas, SKAU-R-6 and DUN6 showed the lowest similarity (0.93) among the genotypes. The dendrogram generated, using Unweighted pair group method using the arithmetic average (UPGMA) from this marker, grouped the 12 silkworm genotypes into five main clusters, which corroborates the differences in their phenotypic characters. Conclusion: The study clearly indicated the potential use of RAPD markers in differentiating silkworm genotypes based on their characters. The information generated on genetically divergent genotypes identified in this study would be useful in future silkworm breeding programmes for modifying the yield potentials of silkworms so as to boost up bivoltine silk production in the interest of temperate sericulture industry.
机译：背景：在育种计划中，根据其表型性状及其DNA水平的遗传变异来选择亲本系是定向育种的更好选择，因为特定的DNA谱图将有助于在其基因组上产生可靠的遗传同一性和多样性估计水平。在这种情况下，随机扩增多态性DNA（RAPD）标记方法被用来确定12种双伏蚕（Bombyx mori L.）基因型之间的遗传多样性和相关性，以鉴定多种基因型作为亲本，以改善其数量性状。蚕。结果：对12种家蚕基因型的RAPD分析显示其高多态性为88.88％。本研究中使用的10种RAPD引物在12种家蚕基因型之间产生了202-2876 bp范围内的PCR产物。多态信息含量（PIC）的范围从0.295到0.493％。根据Nei的相似系数，所研究的蚕基因型之间的遗传距离为0.93至0.99。在成对组合中，CSR 18 和SH 6 的相似性最高（0.99），而SKAU-R-6和DUN 6 的相似性最高。基因型之间的最低相似度（0.93）。使用该标记的算术平均值（UPGMA）使用非加权对分组法生成的树状图将12种蚕基因型分为五个主要簇，这证实了它们的表型特征的差异。结论：该研究清楚地表明，RAPD标记物可根据其特征区分蚕种基因型。在这项研究中鉴定出的有关基因不同基因型的信息将在未来的家蚕育种计划中有用，以改变家蚕的产量潜力，从而提高温带蚕桑业的双伏蚕丝产量。

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