• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >Isolation and Identification of Cellulytic Fungi fromAgrowastes and Sawmill Soils
【24h】

Isolation and Identification of Cellulytic Fungi fromAgrowastes and Sawmill Soils

机译：垃圾和锯木厂土壤中纤维素分解真菌的分离与鉴定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Five agricultural wastes and sawmill soil were investigated for fungi capable of producing cellulase. Isolation was carried using serial dilution techniques and isolates were screened for cellulase production on carboxymethyl cellulose-containing agar plates. Copious isolates were further subjected to submerged cultivation for quantitative evaluation of cellulase biosynthesis. Highest fungal load was 6.67±0.33×106 sfu/g (Sawmill soil) and the least was 2.33±0.33×106 sfu/g (yam peels). A total of sixty seven fungi were identified from the samples. The isolates were of fifteen fungal species namely Mucor mucedo, Aspergillus niger, A. repens, A. flavus, A. parasiticus, Articulospora inflata, Gonatobotrys simplex, Gyrothrix circinata, Dendrospora erecta, Penicillium notatum, P. italicum, Varicosporium elodea, Gonatobotryum apicolatum, Mucor racemosus and Rhizopus nigricans. Aspergillus had the highest occurrence (36.11%), while the least occurrence was Varicosporium and Gonatobotryum (2.78%). Highest cellulase activity ratio was exhibited by Aspergillus niger (1.90) and the least by Aspergillus repens (1.04) on plate screening. The highest cellulase producer among selected isolates under submerged growth was Gonatobotrys simplex (1.2143±0.02 U/ml), followed by Aspergillus niger (1.1429±0.01 U/ml) and the least by Aspergillus parasiticus (0.8265±0.01 U/ml). Submerged protein content was highest in Aspergillus niger culture and least in Penicillium notatum. The study has revealed array of cellulytic fungi from agricultural wastes and sawmill soil.
机译：调查了五种农业废料和锯木厂土壤中能够产生纤维素酶的真菌。使用连续稀释技术进行分离，并在含有羧甲基纤维素的琼脂平板上筛选分离物的纤维素酶生产。将丰富的分离株进一步进行深层培养，以定量评估纤维素酶的生物合成。最高的真菌载量为6.67±0.33×106 sfu / g（锯木厂土壤），最小的为2.33±0.33×106 sfu / g（山药皮）。从样品中鉴定出总共六十七种真菌。分离物有15种真菌，分别是Mucor mucedo，黑曲霉，A。repens，A。flavus，A。parasiticus，Articulospora inflata，Gonatobotrys simplex，Gyrothrix circinata，Dendrospora erecta，noticillium G. ，Mucor racemosus和Rhizopus nigricans。曲霉菌的发生率最高（36.11％），而曲霉菌和性腺菌的发生率最低（2.78％）。在平板筛查中，黑曲霉（1.90）表现出最高的纤维素酶活性比，黑曲霉（1.04）表现出最低的纤维素酶活性比。在淹没生长下所选菌株中，最高的纤维素酶生产者是单性变种（1.2143±0.02 U / ml），其次是黑曲霉（1.1429±0.01 U / ml），最少的是寄生曲霉（0.8265±0.01 U / ml）。在黑曲霉培养物中，浸没的蛋白质含量最高，而在青霉中则最低。该研究揭示了来自农业废料和锯木厂土壤中的一系列纤维素分解真菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号