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首页> 外文期刊>British Biotechnology Journal >Investigating the In vitro Regeneration Potentialityof Three High Yielding Indica Rice (Oryza sativa L.)Varieties
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Investigating the In vitro Regeneration Potentialityof Three High Yielding Indica Rice (Oryza sativa L.)Varieties

机译：三种高产In稻品种的离体再生潜力研究

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Aims: An efficient and reproducible In vitro regeneration protocol is vital for varietal improvement research. The current research was conducted to optimize the callus induction, shoot and root regeneration of three indica rice varieties.Place, Duration and Design of Study: The experiment was conducted in the tissue culture and biotechnology laboratory of the department of Genetics and Plant Breeding of Bangladesh Agricultural University using completely randomized experimental design.Methodology: Dehusked mature seeds of three indica rice varieties namely, BRRI dhan28, BRRI dhan29 and BINA dhan6 were cultured In vitro in MS medium supplemented with different concentrations and combinations of phytohormones.Results: The callus induction ranged from 14 - 84% which showed a general increasing trend with the increase in the concentration of 2,4-Dichlorophenoxyacetic acid (2,4-D) starting from 1.0 mg L-1 till 2.5 mg L-1. A further increase in the concentration of 2,4-D to 2.5 mg L-1, however, decreased the percentage of callus induction in all three varieties. MS medium supplemented with 2.5 mg L-1 2,4-D and 0.5 mg L-1 6-Benzylaminopurine (BAP) was better than any other composition for callus induction. For size of callus and nature of callus, however, MS medium supplemented with 2.0 mg L-1 2,4-D and 0.5 mg L-1 BAP was found to perfume best. The highest percentage of callus induction was observed in the variety BRRI dhan29 (84%) followed by BRRI dhan28 (74%). Almost all the varieties produced yellowish and compact calli except BINA dhan6 which produced creamy and friable calli. The desiccation treatment has shown to increase size but decrease the compactness of the callus. The differences are, however, not statistically significant. MS medium supplemented with 0.6 mg L-1 1-Naphthaleneacetic acid (NAA) and 6 mg L-1 Kinetin (Kn) showed highest shoot regeneration in BRRI dhan29 (85%) followed by BRRI dhan28 (60%). Higher frequency of root formation was observed in all three varieties using Indole-3-butyric acid (IBA). The survival rate of the plantlet in acclimatization chamber (96%) and in field condition (93.33%) was higher for BRRI dhan29. BINA dhan6 has shown the least regeneration potentiality for all the aforementioned traits.Conclusion: Of the three varieties, BRRI dhan29 and BRRI dhan28 has shown higher regeneration potentiality. This optimized protocol will thus be useful in genetic improvement of these varieties using biotechnological manipulations.
机译：目的：有效且可重现的体外再生方案对于品种改良研究至关重要。目前的研究是为了优化三个in稻品种的愈伤组织诱导，芽和根再生。研究的地点，持续时间和设计：该实验在孟加拉国遗传与植物育种系的组织培养和生物技术实验室进行。农业大学采用完全随机的实验设计方法。方法：在补充了不同浓度和植物激素组合的MS培养基中体外培养三个in稻品种BRRI dhan28，BRRI dhan29和BINA dhan6的去壳成熟种子。结果：愈伤组织的诱导范围不等从14-84％，这显示出总体上的增加趋势，其从2，4-二氯苯氧基乙酸（2，4-D）的浓度从1.0mg L-1开始增加到2.5mg L-1。然而，将2，4-D的浓度进一步提高至2.5 mg L-1，却降低了所有三个品种中愈伤组织的诱导百分比。补充有2.5 mg L-1 2,4-D和0.5 mg L-1 6-苄基氨基嘌呤（BAP）的MS培养基优于任何其他诱导愈伤组织的组合物。然而，对于愈伤组织的大小和愈伤组织的性质，发现补充有2.0 mg L-1 2,4-D和0.5 mg L-1 BAP的MS培养基散发着最佳香气。在品种BRRI dhan29（84％）中观察到愈伤组织诱导的最高百分比，其次是BRRI dhan28（74％）。除BINA dhan6产生奶油和脆性愈伤组织外，几乎所有品种均产生淡黄色和紧密的愈伤组织。干燥处理已显示出增大了尺寸，但降低了愈伤组织的致密性。但是，差异在统计上并不显着。补充有0.6 mg L-1 1-萘乙酸（NAA）和6 mg L-1 Kinetin（Kn）的MS培养基在BRRI dhan29（85％）中显示最高的芽再生，其次是BRRI dhan28（60％）。使用吲哚-3-丁酸（IBA）在所有三个品种中均观察到较高的根形成频率。对于BRRI dhan29，小苗在适应室内的存活率（96％）和田间条件下的存活率（93.33％）更高。 BINA dhan6表现出上述所有性状的最低再生潜力。结论：在三个品种中，BRRI dhan29和BRRI dhan28表现出更高的再生潜力。因此，这种优化的方案将有助于通过生物技术操作对这些品种进行遗传改良。

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