首页> 外文期刊>British Biotechnology Journal >Domain Analysis and Isolation of ConiferylAlcohol Dehydrogenase Gene fromPseudomonas nitroreducens Jin1

【24h】

Domain Analysis and Isolation of ConiferylAlcohol Dehydrogenase Gene fromPseudomonas nitroreducens Jin1

机译：硝酸假单胞菌Jin1针叶树醇脱氢酶基因的结构域分析与分离

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Aim: The Aim of present study is to analyse conserved functional Short Dehydrogenase Reductase (SDR) domain from bacteria. Based on the domain analysis selection of coniferyl alcohol dehydrogenase gene for isolation from Pseudomonas nitroreducens Jin1.Place and Duration of Study: Department of Biotechnology, Punjabi University, Patiala. From July, 2012 to November, 2012.Methodology: Bioinformatics tools were used to analyse various calA genes from bacteria based on the presence of conserved domain in members of SDR family. Based on insilico analysis, Pseudomonas nitroreducens Jin1 calA was selected. PCR was used for amplification of the gene from the genome of Pseudomonas nitroreducens Jin1. Result: Multiple sequence alignment results for conserved domains amongst members of SDR family identified presence of all domains of Short Dehydrogenase Reductase members in Pseudomonas nitroreducens Jin1 calA gene. Amongst the various sequences compared the P. nitroreducens Jin1, calA was found to be the smallest in size. The locus of calA in the genome resides at 103513-104280 bases. It was amplified from the genome of Pseudomonas nitroreducens Jin1. The calA gene that was amplified is of size 768bp. Conclusion: calA gene isolated from Pseudomonas nitroreducens Jin1 is a small gene with all the functional domains and can be used for biotransformation of coniferyl alcohol to coniferyl aldehyde.

机译：目的：本研究的目的是分析细菌中保守的功能性短脱氢酶还原酶（SDR）结构域。基于结构域分析，选择了从硝酸假单胞菌中分离得到的松柏醇脱氢酶基因。研究地点和持续时间：旁遮普大学生物技术系，帕蒂亚拉。 2012年7月至2012年11月。方法：根据SDR家族成员中保守域的存在，使用生物信息学工具分析细菌中的各种calA基因。根据计算机分析，选择了硝酸假单胞菌Jin1 calA。 PCR用于从硝酸假单胞菌Jin1的基因组中扩增该基因。结果：SDR家族成员之间的保守域的多个序列比对结果确定了硝化假单胞菌Jin1 calA基因中短脱氢酶还原酶成员的所有域的存在。在比较的各种序列中，硝化疟原虫Jin1中，发现calA的大小最小。基因组中的calA基因座位于103513-104280个碱基。它是从硝酸假单胞菌Jin1的基因组中扩增得到的。扩增的calA基因大小为768bp。结论：硝酸假单胞菌Jin1分离得到的calA基因是一个具有所有功能域的小基因，可用于从松柏醇向松柏醛的生物转化。

著录项

来源
《British Biotechnology Journal》 |2014年第6期|共12页
作者
Praveen P. Balgir; Dinesh Kalra;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类生物工程学（生物技术）;
关键词

相似文献

外文文献
中文文献
专利

1. Isolation and sequencing of the HMG domains of fifteen Sox genes from Hyla sanchiangensis, and analysis of the evolutionary behaviors of Sox duplicated copies based on bioinformatics [J] . CHEN Qi-long, QIAO Zi-jun, CHEN Jian, African Journal of Microbiology Research . 2012,第23期

机译：三叉戟中15个Sox基因HMG结构域的分离，测序及基于生物信息学的Sox重复拷贝进化行为分析
2. A comparative analysis of translated dihydropyrimidine dehydrogenase cDNA; conservation of functional domains and relevance to genetic polymorphisms. [J] . Mattison LK, Johnson MR, Diasio RB Pharmacogenetics . 2002,第2期

机译：翻译的二氢嘧啶脱氢酶cDNA的比较分析;功能域的保守性和与遗传多态性的相关性。
3. Isolation, DNA sequence analysis, and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum. [J] . P F Straub, P H Reynolds, S Althomsons, Applied and Environmental Microbiology . 1996,第1期

机译：日本根瘤菌（Bradyrhizobium japonicum）脯氨酸脱氢酶基因（putA）的分离，DNA序列分析和诱变。
4. Isolation and Characterization of Full-length Putative Alcohol dehydrogenase Genes from Polygonum minus [C] . Nur Athirah Abd Hamid, Ismanizan Ismail UKM FST Postgraduate Colloquium . 2013

机译：多长度调用醇脱氢酶基因的分离与表征型粒子含量
5. Functional consequences of substitutions for the prosthetic group of the inner lipoyl domain of dihydrolipoyl acetyltransferase and the inner lipoyl domain's mechanism of activation of pyruvate dehydrogenase kinase isoform 3 [D] . Peng, Tao. 2001

机译：取代二氢脂酰乙酰基转移酶的内部脂酰结构域的修复基的功能性后果和内部脂酰结构域的丙酮酸脱氢酶激酶同工型3的激活机制
6. Isolation DNA sequence analysis and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum. [O] . P F Straub, P H Reynolds, S Althomsons, 1996

机译：日本根瘤菌（Bradyrhizobium japonicum）脯氨酸脱氢酶基因（putA）的分离DNA序列分析和诱变。
7. Isolation, DNA sequence analysis, and mutagenesis of a proline dehydrogenase gene (putA) from Bradyrhizobium japonicum [O] . P F Straub, P H Reynolds, S Althomsons, 1996

机译：来自Bradyrhizobium japonicum的脯氨酸脱氢酶基因（Puta）的分离，DNA序列分析和诱变
8. Transfer and Expression of an Organophosphate Insecticide-Degrading Gene fromPseudomonas in Drosophila melanogaster [R] . Phillips, J. P., Xin, J. H., Kirby, K., 1990

机译：黑腹果蝇中假单胞菌有机磷杀线虫降解基因的转移和表达

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

客服微信
服务号