...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Molecular Biology >Efficient 5′-3′ DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicus
【24h】

Efficient 5′-3′ DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicus

机译：HerA和NurA进行有效的5'-3'DNA末端切除对于在火棘鱼Sulfolobus islandicus中的细胞活力至关重要

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hyperthermophilic archaeon, as a model to investigate the in vivo functions of the ATPase/helicase HerA, the nuclease NurA, and their associated proteins Mre11 and Rad50. We revealed that each of the four genes in the same operon, mre11, rad50, herA, and nurA, are essential for cell viability by a mutant propagation assay. A genetic complementation assay with mutant proteins was combined with biochemical characterization demonstrating that the ATPase activity of HerA, the interaction between HerA and NurA, and the efficient 5′-3′ DNA end resection activity of the HerA-NurA complex are essential for cell viability. NurA and two other putative HRR proteins: a PIN (PilT N-terminal)-domain containing ATPase and the Holliday junction resolvase Hjc, were co-purified with a chromosomally encoded N-His-HerA in vivo. The interactions of HerA with the ATPase and Hjc were further confirmed by in vitro pull down. Efficient 5′-3′ DNA end resection activity of the HerA-NurA complex contributes to necessity of HerA and NurA in Sulfolobus, which is crucial to yield a 3′-overhang in HRR. HerA may have additional binding partners in cells besides NurA.
机译：ATPase /螺旋酶和核酸酶在同源重组修复（HRR）中起重要作用。与这些酶有关的许多机制细节及其在这种基本而复杂的DNA修复过程中的作用在古细菌中仍然知之甚少。在这里，我们采用了嗜热古细菌Sulfolobus islandicus，作为模型来研究ATPase /解旋酶HerA，核酸酶NurA及其相关蛋白Mre11和Rad50的体内功能。我们揭示了通过突变繁殖试验，同一操纵子中的四个基因mre11，rad50，herA和nurA中的每个基因对于细胞活力都是必不可少的。突变蛋白的遗传互补分析与生化特征相结合，证明了HerA的ATPase活性，HerA与NurA之间的相互作用以及HerA-NurA复合物的有效5'-3'DNA末端切除活性对于细胞活力至关重要。 NurA和其他两个假定的HRR蛋白：含有ATPase的PIN（PilT N末端）结构域和Holliday连接分辨酶Hjc在体内与染色体编码的N-His-HerA共纯化。通过体外下拉进一步证实了HerA与ATPase和Hjc的相互作用。 HerA-NurA复合物的有效5'-3'DNA末端切除活性有助于在Sulfolobus中获得HerA和NurA，这对于在HRR中产生3'突出端至关重要。除了NurA，HerA在细胞中可能还有其他结合伴侣。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号