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首页> 外文期刊>BMC Microbiology >MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
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MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

机译：来自不同动物和地理起源的295种海洋哺乳动物布鲁氏菌分离株的MLVA-16分型可识别布鲁氏菌ceti和布鲁氏菌属的7个主要群体

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Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. Results 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals (Cystophora cristata) and the two others comprising other seal species isolates. Conclusion The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest of the Brucella genus. MLVA-16 is confirmed as being a rapid, highly discriminatory and reproducible method to classify Brucella strains including the marine mammal isolates. The Brucella2009 MLVA-16 genotyping database available at http://mlva.u-psud.fr/ webcite is providing a detailed coverage of all 9 currently recognized Brucella species.
机译：背景技术自1994年以来，布鲁氏菌菌株已从多种海洋哺乳动物中分离出来。它们目前被公认为是两种新的布鲁氏菌属，与寄主偏好以及特定的表型和分子标记相符，即针对钉状分离物的B. pinnipedialis和针对鲸蜡类分离物的B. ceti。为了调查海洋哺乳动物布鲁氏菌分离株内的遗传关系，并参考陆地哺乳动物布鲁氏菌分离株，我们在本研究中应用了多基因座VNTR（串联重复序列可变数）分析（MLVA）方法。使用了先前公布的包含16个基因座（MLVA-16）的测定法，该测定法已被证明与从动物和人类起源的布鲁氏菌菌株的分型和聚类高度相关且有效。结果用MLVA-16研究了欧洲水域收集的294种海洋哺乳动物布鲁氏菌菌株，其中包括173种动物和一种来自新西兰的人类分离株。已显示海洋哺乳动物布鲁氏菌分离株不同于公认的陆地哺乳动物布鲁氏菌物种和生物变种，并对应于3个主要的相关群体，其中一个是B. ceti菌株，一个是B. pinnipedialis菌株，最后一个由人分离株组成。在盲肠芽孢杆菌组中，鉴定出3个亚群，区分出海豚，小须鲸和海豚分离物的簇和两个主要由海豚分离物组成的簇。这些结果与使用其他表型或分子方法或VNTR基因座的不同面板发表的分析一致。 B. pinnipedialis组可以类似地细分为3个亚群，一个亚群仅由带帽海豹（Cystophora cristata）的分离物组成，另外两个由其他海豹物种的分离物组成。结论对来自欧洲水域的大量海洋哺乳动物布鲁氏菌分离株的聚类分析显着增强了目前对这两个物种的种群结构及其相对于布鲁氏菌属其他物种的相对位置的看法。 MLVA-16被证实是对布鲁氏菌菌株（包括海洋哺乳动物分离株）进行分类的一种快速，高度歧视性和可重复性的方法。网站http://mlva.u-psud.fr/上提供的Brucella2009 MLVA-16基因分型数据库可提供有关所有9种目前公认的Brucella物种的详细报道。

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