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首页> 外文期刊>BMC Microbiology >Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium
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Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium

机译：肠炎沙门氏菌鼠伤寒沙门氏菌PreA / PreB（QseB / QseC）调节子的全基因组分析

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Background The Salmonella PreA/PreB two-component system (TCS) is an ortholog of the QseBC TCS of Escherichia coli. In both Salmonella and E. coli, this system has been shown to affect motility and virulence in response to quorum-sensing and hormonal signals, and to affect the transcription of the Salmonella enterica serovar Typhimurium (S. Typhimurium) pmrAB operon, which encodes an important virulence-associated TCS. Results To determine the PreA/PreB regulon in S. Typhimurium, we performed DNA microarrays comparing the wild type strain and various preA and/or preB mutants in the presence of ectopically expressed preA (qseB). These data confirmed our previous findings of the negative effect of PreB on PreA gene regulation and identified candidate PreA-regulated genes. A proportion of the activated loci were previously identified as PmrA-activated genes (yibD, pmrAB, cptA, etc.) or were genes located in the local region around preA, including the preAB operon. The transcriptional units were defined in this local region by RT-PCR, suggesting three PreA activated operons composed of preA-preB, mdaB-ygiN, and ygiW-STM3175. Several putative virulence-related phenotypes were examined for preAB mutants, resulting in the observation of a host cell invasion and slight virulence defect of a preAB mutant. Contrary to previous reports on this TCS, we were unable to show a PreA/PreB-dependent effect of the quorum-sensing signal AI-2 or of epinephrine on S. Typhimurium with regard to bacterial motility. Conclusion This work further characterizes this unorthadox OmpR/EnvZ class TCS and provides novel candidate regulated genes for further study. This first in-depth study of the PreA/PreB regulatory system phenotypes and regulation suggests significant comparative differences to the reported function of the orthologous QseB/QseC in E. coli.
机译：背景沙门氏菌PreA / PreB两组分系统（TCS）是大肠杆菌QseBC TCS的直系同源物。在沙门氏菌和大肠杆菌中，该系统已显示出对群体感应和激素信号的反应会影响运动性和毒力，并影响肠炎沙门氏菌鼠伤寒沙门氏菌（S. Typhimurium）pmrAB操纵子的转录，该编码子重要的毒力相关TCS。结果为了确定鼠伤寒沙门氏菌中的PreA / PreB调节子，我们进行了DNA微阵列比较，比较了野生型菌株和异位表达的preA（qseB）存在的各种preA和/或preB突变体。这些数据证实了我们先前关于PreB对PreA基因调控的负面影响的发现，并确定了候选的PreA调控基因。先前已将一部分激活基因座鉴定为PmrA激活基因（yibD，pmrAB，cptA等），或者是位于preA周围局部区域（包括preAB操纵子）的基因。通过RT-PCR在此局部区域中定义了转录单位，这表明三个由preA-preB，mdaB-ygiN和ygiW-STM3175组成的PreA激活操纵子。检查了几种假定的毒力相关表型以检测preAB突变体，从而观察到宿主细胞入侵和preAB突变体的轻微毒力缺陷。与以前有关该TCS的报道相反，我们未能显示出在细菌运动性方面，群体感应信号AI-2或肾上腺素对鼠伤寒沙门氏菌具有PreA / PreB依赖性作用。结论这项工作进一步表征了这种非胸膜OmpR / EnvZ类TCS，并为新的候选调控基因提供了进一步的研究。对PreA / PreB调控系统表型和调控的首次深入研究表明，与报道的直系同源QseB / QseC在大肠杆菌中的功能相比存在显着的比较差异。

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