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首页> 外文期刊>BMC Microbiology >Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis
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Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis

机译：长/短菌毛和牙龈蛋白酶在牙龈卟啉单胞菌同型生物膜发育中的不同作用

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Background Porphyromonas gingivalis, a periodontal pathogen, expresses a number of virulence factors, including long (FimA) and short (Mfa) fimbriae as well as gingipains comprised of arginine-specific (Rgp) and lysine-specific (Kgp) cysteine proteinases. The aim of this study was to examine the roles of these components in homotypic biofilm development by P. gingivalis, as well as in accumulation of exopolysaccharide in biofilms. Results Biofilms were formed on saliva-coated glass surfaces in PBS or diluted trypticase soy broth (dTSB). Microscopic observation showed that the wild type strain formed biofilms with a dense basal monolayer and dispersed microcolonies in both PBS and dTSB. A FimA deficient mutant formed patchy and small microcolonies in PBS, but the organisms proliferated and formed a cohesive biofilm with dense exopolysaccharides in dTSB. A Mfa mutant developed tall and large microcolonies in PBS as well as dTSB. A Kgp mutant formed markedly thick biofilms filled with large clumped colonies under both conditions. A RgpA/B double mutant developed channel-like biofilms with fibrillar and tall microcolonies in PBS. When this mutant was studied in dTSB, there was an increase in the number of peaks and the morphology changed to taller and loosely packed biofilms. In addition, deletion of FimA reduced the autoaggregation efficiency, whereas autoaggregation was significantly increased in the Kgp and Mfa mutants, with a clear association with alteration of biofilm structures under the non-proliferation condition. In contrast, this association was not observed in the Rgp-null mutants. Conclusion These results suggested that the FimA fimbriae promote initial biofilm formation but exert a restraining regulation on biofilm maturation, whereas Mfa and Kgp have suppressive and regulatory roles during biofilm development. Rgp controlled microcolony morphology and biovolume. Collectively, these molecules seem to act coordinately to regulate the development of mature P. gingivalis biofilms.
机译：背景牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种牙周病原体，表达多种毒力因子，包括长（FimA）和短（Mfa）菌毛以及由精氨酸特异性（Rgp）和赖氨酸特异性（Kgp）半胱氨酸蛋白酶组成的姜黄素。这项研究的目的是检查这些成分在牙龈卟啉单胞菌同型生物膜发育以及生物膜中胞外多糖积累中的作用。结果在PBS或稀释的胰蛋白tic大豆肉汤（dTSB）中，唾液覆盖的玻璃表面上形成了生物膜。显微镜观察表明，野生型菌株形成了具有致密的基底单层和分散在PBS和dTSB中的微菌落的生物膜。 FimA缺陷突变体在PBS中形成了斑块状的小菌落，但生物体增殖并在dTSB中形成了具有密集胞外多糖的粘性生物膜。一个Mfa突变体在PBS和dTSB中形成了高大的微菌落。在两种条件下，Kgp突变体均形成了明显厚的生物膜，充满了大块的菌落。 RgpA / B双突变体在PBS中形成了具有纤维状和高菌落的通道状生物膜。当在dTSB中研究此突变体时，峰数增加并且形态变为更高且松散堆积的生物膜。另外，FimA的缺失降低了自动聚集效率，而在Kgp和MFa突变体中，自动聚集显着增加，这与在非增殖条件下生物膜结构的改变明显相关。相反，在Rgp-null突变体中未观察到这种关联。结论这些结果表明，FimA菌毛促进了初始生物膜形成，但对生物膜成熟具有抑制作用，而Mfa和Kgp在生物膜发育过程中具有抑制和调节作用。 Rgp控制的小菌落形态和生物量。总的来说，这些分子似乎协调地调节成熟的牙龈卟啉单胞菌生物膜的发育。

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