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首页> 外文期刊>BMC Microbiology >The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis
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The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis

机译：低亲和力磷酸盐转运蛋白PitA可用于耻垢分枝杆菌的体外生长

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Background Mycobacteria have been shown to contain an apparent redundancy of high-affinity phosphate uptake systems, with two to four copies of such systems encoded in all mycobacterial genomes sequenced to date. In addition, all mycobacteria also contain at least one gene encoding the low-affinity phosphate transporter, Pit. No information is available on a Pit system from a Gram-positive microorganism, and the importance of this system in a background of multiple other phosphate transporters is unclear. Results The aim of this study was to determine the physiological role of the PitA phosphate transporter in Mycobacterium smegmatis. Expression of pitA was found to be constitutive under a variety of growth conditions. An unmarked deletion mutant in pitA of M. smegmatis was created. The deletion did not affect in vitro growth or phosphate uptake of M. smegmatis. Expression of the high-affinity transporters, PstSCAB and PhnDCE, was increased in the pitA deletion strain. Conclusion PitA is the only low-affinity phosphate transport system annotated in the genome of M. smegmatis. The lack of phenotype of the pitA deletion strain shows that this system is dispensable for in vitro growth of this organism. However, increased expression of the remaining phosphate transporters in the mutant indicates a compensatory mechanism and implies that PitA is indeed used for the uptake of phosphate in M. smegmatis.
机译：背景技术已证明分枝杆菌含有明显的高亲和力磷酸盐摄取系统冗余，迄今已测序的所有分枝杆菌基因组中编码了这种系统的2-4个拷贝。另外，所有分枝杆菌还包含至少一种编码低亲和力磷酸盐转运蛋白Pit的基因。没有关于革兰氏阳性微生物的Pit系统的信息，并且该系统在多种其他磷酸盐转运蛋白的背景下的重要性尚不清楚。结果本研究的目的是确定PitA磷酸转运蛋白在耻垢分枝杆菌中的生理作用。发现pitA的表达在多种生长条件下是组成型的。在耻垢分枝杆菌的pitA中创建了未标记的缺失突变体。该删除不影响耻垢分枝杆菌的体外生长或磷酸盐吸收。在pitA缺失菌株中，高亲和力转运蛋白PstSCAB和PhnDCE的表达增加。结论PitA是耻垢分枝杆菌基因组中唯一注释的低亲和力磷酸盐转运系统。缺少pitA缺失菌株的表型表明该系统对于该生物的体外生长是可有可无的。但是，突变体中其余磷酸盐转运蛋白的表达增加表明有补偿机制，这暗示着PitA确实被用于耻垢分枝杆菌中的磷酸盐吸收。

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