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首页> 外文期刊>BMC Molecular Biology >Temperature-induced variation in gene expression burst size in metazoan cells
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Temperature-induced variation in gene expression burst size in metazoan cells

机译：温度诱导后生细胞基因表达爆发大小的变化

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摘要

Gene expression is an inherently stochastic process, owing to its dynamic molecular nature. Protein amount distributions, which can be acquired by cytometry using a reporter gene, can inform about the mechanisms of the underlying microscopic molecular system. By using different clones of chicken erythroid progenitor cells harboring different integration sites of a CMV-driven mCherry protein, we investigated the dynamical behavior of such distributions. We show that, on short term, clone distributions can be quickly regenerated from small population samples with a high accuracy. On longer term, on the contrary, we show variations manifested by correlated fluctuation in the Mean Fluorescence Intensity. In search for a possible cause of this correlation, we demonstrate that in response to small temperature variations cells are able to adjust their gene expression rate: a modest (2 °C) increase in external temperature induces a significant down regulation of mean expression values, with a reverse effect observed when the temperature is decreased. Using a two-state model of gene expression we further demonstrate that temperature acts by modifying the size of transcription bursts, while the burst frequency of the investigated promoter is less systematically affected. For the first time, we report that transcription burst size is a key parameter for gene expression that metazoan cells from homeotherm animals can modify in response to an external thermal stimulus.
机译：由于其动态分子性质，基因表达是固有的随机过程。可以通过使用报告基因的细胞术获得的蛋白质数量分布，可以告知潜在的微观分子系统的机制。通过使用包含CMV驱动的mCherry蛋白的不同整合位点的鸡类红细胞祖细胞的不同克隆，我们研究了这种分布的动力学行为。我们表明，从短期来看，克隆分布可以从小型种群样本中快速准确地再生。相反，从长远来看，我们显示出以平均荧光强度的相关波动为特征的变化。在寻找这种相关性的可能原因时，我们证明了响应较小的温度变化，细胞能够调节其基因表达率：外部温度的适度（2°C）升高会导致平均表达值的显着下调，当温度降低时，观察到相反的效果。使用基因表达的两种状态模型，我们进一步证明了温度通过修饰转录爆发的大小起作用，而所研究的启动子的爆发频率受到的系统影响较小。首次，我们报道转录爆发大小是基因表达的关键参数，来自恒温动物的后生动物可以响应外部热刺激而对其进行修饰。

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