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首页> 外文期刊>BMC Neuroscience >Astroglial-axonal interactions during early stages of myelination in mixed cultures using in vitro and ex vivo imaging techniques
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Astroglial-axonal interactions during early stages of myelination in mixed cultures using in vitro and ex vivo imaging techniques

机译：使用体外和离体成像技术在混合培养的髓鞘形成早期的星形胶质-轴突相互作用

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Backgound Myelination is a very complex process that requires the cross talk between various neural cell types. Previously, using cytosolic or membrane associated GFP tagged neurospheres, we followed the interaction of oligodendrocytes with axons using time-lapse imaging in vitro and ex vivo and demonstrated dynamic changes in cell morphology. In this study we focus on GFP tagged astrocytes differentiated from neurospheres and their interactions with axons. Results We show the close interaction of astrocyte processes with axons and with oligodendrocytes in mixed mouse spinal cord cultures with formation of membrane blebs as previously seen for oligodendrocytes in the same cultures. When GFP-tagged neurospheres were transplanted into the spinal cord of the dysmyelinated shiverer mouse, confirmation of dynamic changes in cell morphology was provided and a prevalence for astrocyte differentiation compared with oligodendroglial differentiation around the injection site. Furthermore, we were able to image GFP tagged neural cells in vivo after transplantation and the cells exhibited similar membrane changes as cells visualised in vitro and ex vivo . Conclusion These data show that astrocytes exhibit dynamic cell process movement and changes in their membrane topography as they interact with axons and oligodendrocytes during the process of myelination, with the first demonstration of bleb formation in astrocytes.
机译：背景髓鞘形成是一个非常复杂的过程，需要各种神经细胞类型之间的串扰。以前，我们使用胞质或膜相关的GFP标记神经球，通过体外和离体延时成像追踪少突胶质细胞与轴突的相互作用，并证明了细胞形态的动态变化。在这项研究中，我们专注于从神经球分化的GFP标记星形胶质细胞及其与轴突的相互作用。结果我们显示了星形胶质细胞过程与轴突和少突胶质细胞在混合小鼠脊髓培养物中的紧密相互作用，并形成了膜泡，如先前在相同培养物中的少突胶质细胞所见。当将带有GFP标签的神经球移植到dysmyelinated颤抖小鼠的脊髓中时，证实了细胞形态的动态变化，并且与注射部位周围少突胶质细胞分化相比，星形胶质细胞的分化普遍存在。此外，我们能够在移植后在体内对GFP标记的神经细胞进行成像，并且这些细胞表现出与体外和离体可见细胞相似的膜变化。结论这些数据表明，星形胶质细胞在髓鞘化过程中与轴突和少突胶质细胞相互作用时表现出动态的细胞过程运动和膜形貌变化，首次证明了星形胶质细胞中形成了气泡。

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