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首页> 外文期刊>BMC Neuroscience >Expression of ionotropic glutamate receptors in the retina of the rdta transgenic mouse
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Expression of ionotropic glutamate receptors in the retina of the rdta transgenic mouse

机译：离子型谷氨酸受体在rdta转基因小鼠视网膜中的表达

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Background The expression of retinal CaMKII is up-regulated in the retina of the rdta mouse in which rod photoreceptors are genetically ablated. As ionotropic glutamate receptors are known substrates of CAMKII, this study set out to determine if the protein levels of ionotropic glutamate receptors in the rdta mouse retina are also affected. Results The NMDA receptor subunits (NR1, NR2A/B) and the GluR1; AMPA receptor subunit (GluR1) were examined in immunolabeled western blots. The results demonstrate that the amounts of NR1 and NR2A/B receptor subunits are significantly increased in crude synaptic membrane fractions isolated from retinae of the rdta mice when compared to their normal, littermate controls. The GluR1 receptor subunit and its phosphorylation are simultaneously increased in retinae of the rdta mice. Conclusions These data indicate that the NMDA receptors and AMPA (GluR1) receptors are altered in the retinae of rdta mice that lack rod photoreceptors. Because the rods are lost at an early stage in development, it is likely that these results are indicative of synaptic reorganization in the retina.
机译：背景技术视网膜杆状感光细胞在遗传上被消融的rdta小鼠视网膜中，视网膜CaMKII的表达上调。由于离子型谷氨酸受体是CAMKII的已知底物，因此该研究着手确定rdta小鼠视网膜中离子型谷氨酸受体的蛋白水平是否也受到影响。结果NMDA受体亚基（NR1，NR2A / B）和GluR1;在免疫标记的蛋白质印迹中检查了AMPA受体亚基（GluR1）。结果表明，与正常，同窝对照相比，从rdta小鼠视网膜分离的粗突触膜级分中的NR1和NR2A / B受体亚基的含量显着增加。 GluR1受体亚基及其磷酸化在rdta小鼠的视网膜中同时增加。结论这些数据表明缺少杆状光感受器的rdta小鼠的视网膜中NMDA受体和AMPA（GluR1）受体发生了改变。由于视杆在发育的早期阶段丢失，因此这些结果很可能表明视网膜中的突触重组。

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