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首页> 外文期刊>BMC Neuroscience >Nerve growth factor selectively regulates expression of transcripts encoding ribosomal proteins
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Nerve growth factor selectively regulates expression of transcripts encoding ribosomal proteins

机译：神经生长因子选择性调节编码核糖体蛋白的转录本的表达

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Background NGF exerts a variety of actions including promotion of neuronal differentiation and survival. The PC12 rat pheochromocytoma cell line has proved valuable for studying how NGF works and has revealed that the NGF mechanism includes regulation of gene expression. Accordingly, we used SAGE (Serial Analysis of Gene Expression) to compare levels of specific transcripts in PC12 cells before and after long-term NGF exposure. Of the approximately 22,000 transcripts detected and quantified, 4% are NGF-regulated by 6-fold or more. Here, we used database information to identify transcripts in our SAGE libraries that encode ribosomal proteins and have compared the effect of NGF on their relative levels of expression. Results Among the transcripts detected in our SAGE analysis, 74 were identified as encoding ribosomal proteins. Ribosomal protein transcripts were among the most abundantly expressed and, for naive and NGF-treated PC12 cells, represented 5.2% and 3.5%, respectively, of total transcripts analyzed. Surprisingly, nearly half of ribosomal protein transcripts underwent statistically significant NGF-promoted alterations in relative abundance, with changes of up to 5-fold. Of the changes, approximately 2/3 represented decreases. A time course revealed that the relative abundance of transcripts encoding RPL9 increases within 1 hr of NGF treatment and is maximally elevated by 8 hr. Conclusions These data establish that NGF selectively changes expression of ribosomal protein transcripts. These findings raise potential roles for regulation of ribosomal protein transcripts in NGF-promoted withdrawal from the cell cycle and neuronal differentiation and indicate that regulation of individual ribosomal protein transcripts is cell- and stimulus-specific.
机译：背景NGF发挥多种作用，包括促进神经元分化和存活。事实证明，PC12大鼠嗜铬细胞瘤细胞系对于研究NGF的工作原理具有重要意义，并揭示了NGF的机制包括基因表达的调控。因此，我们使用SAGE（基因表达的序列分析）来比较长期NGF暴露前后PC12细胞中特定转录本的水平。在大约22,000个被检测和量化的转录本中，有4％被NGF调节了6倍或更多。在这里，我们使用数据库信息来识别SAGE文库中的编码核糖体蛋白的转录本，并比较了NGF对它们相对表达水平的影响。结果在我们的SAGE分析中检测到的转录本中，有74个被鉴定为编码核糖体蛋白。核糖体蛋白转录物是表达最丰富的蛋白质之一，对于幼稚和经NGF处理的PC12细胞，分别占所分析总转录物的5.2％和3.5％。出人意料的是，近一半的核糖体蛋白转录本经历了NGF促进的相对丰度的统计学显着变化，变化高达5倍。在这些变化中，大约2/3表示减少。一个时间过程显示，在NGF处理的1小时内，编码RPL9的转录本的相对丰度增加，最大增加8小时。结论这些数据证明NGF选择性地改变了核糖体蛋白转录本的表达。这些发现提高了调节核糖体蛋白转录物在NGF促进的细胞周期退出和神经元分化中的潜在作用，并表明单个核糖体蛋白转录物的调节是细胞和刺激特异性的。

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