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首页> 外文期刊>BMC Neuroscience >Recording long-term potentiation of synaptic transmission by three-dimensional multi-electrode arrays
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Recording long-term potentiation of synaptic transmission by three-dimensional multi-electrode arrays

机译：通过三维多电极阵列记录突触传递的长期增强

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Background Multi-electrode arrays (MEAs) have become popular tools for recording spontaneous and evoked electrical activity of excitable tissues. The majority of previous studies of synaptic transmission in brain slices employed MEAs with planar electrodes that had limited ability to detect signals coming from deeper, healthier layers of the slice. To overcome this limitation, we used three-dimensional (3D) MEAs with tip-shaped electrodes to probe plasticity of field excitatory synaptic potentials (fEPSPs) in the CA1 area of hippocampal slices of 129S5/SvEvBrd and C57BL/6J-TyrC-Brd mice. Results Using 3D MEAs, we were able to record larger fEPSPs compared to signals measured by planar MEAs. Several stimulation protocols were used to induce long-term potentiation (LTP) of synaptic responses in the CA1 area recorded following excitation of Sch?ffer collateral/commissural fibres. Either two trains of high frequency tetanic stimulation or three trains of theta-burst stimulation caused a persistent, pathway specific enhancement of fEPSPs that remained significantly elevated for at least 60 min. A third LTP induction protocol that comprised 150 pulses delivered at 5 Hz, evoked moderate LTP if excitation strength was increased to 1.5× of the baseline stimulus. In all cases, we observed a clear spatial plasticity gradient with maximum LTP levels detected in proximal apical dendrites of pyramidal neurones. No significant differences in the manifestation of LTP were observed between 129S5/SvEvBrd and C57BL/6J-TyrC-Brd mice with the three protocols used. All forms of plasticity were sensitive to inhibition of N -methyl- D -aspartate (NMDA) receptors. Conclusion Principal features of LTP (magnitude, pathway specificity, NMDA receptor dependence) recorded in the hippocampal slices using MEAs were very similar to those seen in conventional glass electrode experiments. Advantages of using MEAs are the ability to record from different regions of the slice and the ease of conducting several experiments on a multiplexed platform which could be useful for efficient screening of novel transgenic mice.
机译：背景技术多电极阵列（MEA）已成为记录可激发组织自发和诱发的电活动的流行工具。先前大多数关于大脑切片中突触传递的研究都使用带有平面电极的MEA，而MEA探测到来自更深，更健康的切片层的信号的能力有限。为克服此限制，我们使用带有尖端形电极的三维（3D）MEA来探测129S5 / SvEvBrd和C57BL / 6J-Tyr 的海马切片CA1区的场兴奋性突触电位（fEPSPs）的可塑性。 C-Brd 小鼠。结果使用3D MEAs，与通过平面MEAs测得的信号相比，我们能够记录更大的fEPSPs。几种刺激方案被用于诱导Schiff侧支/连合纤维激发后在CA1区域引起的突触反应的长期增强（LTP）。两列高频强直性刺激或三列theta爆发刺激导致fEPSP持续，途径特异性增强，并持续至少60分钟显着升高。如果激发强度增加到基线刺激的1.5倍，第三个LTP感应协议包括150个在5 Hz处传递的脉冲，会引起中等LTP。在所有情况下，我们观察到明显的空间可塑性梯度，在锥体神经元的近端根尖树突中检测到最大LTP水平。在使用三种方案的129S5 / SvEvBrd和C57BL / 6J-Tyr C-Brd 小鼠之间，LTP的表现没有显着差异。所有形式的可塑性都对抑制N-甲基-D-天冬氨酸（NMDA）受体敏感。结论使用MEAs在海马切片中记录的LTP的主要特征（幅度，途径特异性，NMDA受体依赖性）与常规玻璃电极实验中观察到的非常相似。使用MEA的优点是能够从切片的不同区域进行记录，并且易于在多重平台上进行多个实验，这对于有效筛选新型转基因小鼠很有用。

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