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首页> 外文期刊>BMC Neuroscience >Cerebrospinal fluid promotes survival and astroglial differentiation of adult human neural progenitor cells but inhibits proliferation and neuronal differentiation
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Cerebrospinal fluid promotes survival and astroglial differentiation of adult human neural progenitor cells but inhibits proliferation and neuronal differentiation

机译：脑脊液促进成年人类神经祖细胞的存活和星形胶质细胞分化，但抑制增殖和神经元分化

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Background Neural stem cells (NSCs) are a promising source for cell replacement therapies for neurological diseases. Growing evidence suggests an important role of cerebrospinal fluid (CSF) not only on neuroectodermal cells during brain development but also on the survival, proliferation and fate specification of NSCs in the adult brain. Existing in vitro studies focused on embryonic cell lines and embryonic CSF. We therefore studied the effects of adult human leptomeningeal CSF on the behaviour of adult human NSCs (ahNSCs). Results Adult CSF increased the survival rate of adult human NSCs compared to standard serum free culture media during both stem cell maintenance and differentiation. The presence of CSF promoted differentiation of NSCs leading to a faster loss of their self-renewal capacity as it is measured by the proliferation markers Ki67 and BrdU and stronger cell extension outgrowth with longer and more cell extensions per cell. After differentiation in CSF, we found a larger number of GFAP+ astroglial cells compared to differentiation in standard culture media and a lower number of β-tubulin III+ neuronal cells. Conclusions Our data demonstrate that adult human leptomeningeal CSF creates a beneficial environment for the survival and differentiation of adult human NSCs. Adult CSF is in vitro a strong glial differentiation stimulus and leads to a rapid loss of stem cell potential.
机译：背景技术神经干细胞（NSC）是用于神经疾病的细胞替代疗法的有希望的来源。越来越多的证据表明，脑脊液（CSF）不仅在大脑发育过程中对神经外胚层细胞具有重要作用，而且对成年脑中NSC的存活，增殖和命运特征也具有重要作用。现有的体外研究集中在胚胎细胞系和胚胎CSF。因此，我们研究了成年人类软脑膜脑脊液对成年人类NSC（ahNSC）行为的影响。结果在干细胞维持和分化过程中，与标准无血清培养基相比，成年CSF提高了成年人NSC的存活率。 CSF的存在促进了NSC的分化，导致NSC自我更新能力的丧失更快，这是通过增殖标记Ki67和BrdU来测量的，以及更强的细胞延伸产物的生长以及每个细胞越来越长的细胞延伸。 CSF分化后，我们发现与标准培养基中的分化相比，GFAP + 星形胶质细胞数量更多，而β-微管蛋白III + 神经元细胞数量更少。结论我们的数据表明，成年人类软脑膜脑脊液为成年人类NSC的存活和分化创造了有利的环境。成人CSF是体外强烈的神经胶质分化刺激，并导致干细胞潜能的快速丧失。

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