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PineappleDB: An online pineapple bioinformatics resource

机译:PineappleDB:在线菠萝生物信息资源

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Background A world first pineapple EST sequencing program has been undertaken to investigate genes expressed during non-climacteric fruit ripening and the nematode-plant interaction during root infection. Very little is known of how non-climacteric fruit ripening is controlled or of the molecular basis of the nematode-plant interaction. PineappleDB was developed to provide the research community with access to a curated bioinformatics resource housing the fruit, root and nematode infected gall expressed sequences. Description PineappleDB is an online, curated database providing integrated access to annotated expressed sequence tag (EST) data for cDNA clones isolated from pineapple fruit, root, and nematode infected root gall vascular cylinder tissues. The database currently houses over 5600 EST sequences, 3383 contig consensus sequences, and associated bioinformatic data including splice variants, Arabidopsis homologues, both MIPS based and Gene Ontology functional classifications, and clone distributions. The online resource can be searched by text or by BLAST sequence homology. The data outputs provide comprehensive sequence, bioinformatic and functional classification information. Conclusion The online pineapple bioinformatic resource provides the research community with access to pineapple fruit and root/gall sequence and bioinformatic data in a user-friendly format. The search tools enable efficient data mining and present a wide spectrum of bioinformatic and functional classification information. PineappleDB will be of broad appeal to researchers investigating pineapple genetics, non-climacteric fruit ripening, root-knot nematode infection, crassulacean acid metabolism and alternative RNA splicing in plants.
机译:背景技术已经进行了世界上第一个菠萝EST测序程序,以研究在非更年期的果实成熟过程中表达的基因以及在根部感染过程中线虫与植物的相互作用。如何控制非更年期的果实成熟或线虫与植物相互作用的分子基础知之甚少。 PineappleDB的开发目的是为研究社区提供经过精心挑选的生物信息学资源,其中包含果实,根和线虫感染的胆汁表达序列。描述PineappleDB是一个在线的在线数据库,为从菠萝果实,根和线虫感染的根胆血管组织分离的cDNA克隆提供对带注释的表达序列标签(EST)数据的集成访问。该数据库当前包含5600多个EST序列,3383个重叠群共有序列以及相关的生物信息学数据,包括剪接变体,拟南芥同源物,基于MIPS和基因本体功能分类以及克隆分布。可以通过文本或BLAST序列同源性搜索在线资源。数据输出提供全面的序列,生物信息学和功能分类信息。结论网上菠萝生物信息资源以方便用户的方式为研究社区提供了菠萝果实和根/胆序列以及生物信息数据的访问权限。搜索工具可以进行有效的数据挖掘,并提供广泛的生物信息学和功能分类信息。 PineappleDB将对研究菠萝遗传学,非更年期果实成熟,根结线虫感染,十字绣科植物酸代谢和植物中其他RNA剪接的研究者具有广泛吸引力。

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