...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Neuroscience >Expression of Hepatoma-derived growth factor family members in the adult central nervous system
【24h】

Expression of Hepatoma-derived growth factor family members in the adult central nervous system

机译：肝癌衍生生长因子家族成员在成人中枢神经系统中的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Hepatoma-derived growth factor (HDGF) belongs to a polypeptide family containing five additional members called HDGF related proteins 1–4 (HRP-1 to -4) and Lens epithelial derived growth factor. Whereas some family members such as HDGF and HRP-2 are expressed in a wide range of tissues, the expression of others is very restricted. HRP-1 and -4 are only expressed in testis, HRP-3 only in the nervous system. Here we investigated the expression of HDGF, HRP-2 and HRP-3 in the central nervous system of adult mice on the cellular level by immunohistochemistry. In addition we performed Western blot analysis of various brain regions as well as neuronal and glial cell cultures. Results HDGF was rather evenly expressed throughout all brain regions tested with the lowest expression in the substantia nigra. HRP-2 was strongly expressed in the thalamus, prefrontal and parietal cortex, neurohypophysis, and the cerebellum, HRP-3 in the bulbus olfactorius, piriform cortex and amygdala complex. HDGF and HRP-2 were found to be expressed by neurons, astrocytes and oligodendrocytes. In contrast, strong expression of HRP-3 in the adult nervous system is restricted to neurons, except for very weak expression in oligodendrocytes in the brain stem. Although the majority of neurons are HRP-3 positive, some like cerebellar granule cells are negative. Conclusion The coexpression of HDGF and HRP-2 in glia and neurons as well as the coexpression of all three proteins in many neurons suggests different functions of members of the HDGF protein family in cells of the central nervous system that might include proliferation as well as cell survival. In addition the restricted expression of HRP-3 point to a special function of this family member for neuronal cells.
机译：背景肝癌衍生生长因子（HDGF）属于多肽家族，包含五个额外成员，称为HDGF相关蛋白1-4（HRP-1至-4）和Lens上皮衍生生长因子。尽管某些家族成员（例如HDGF和HRP-2）在广泛的组织中表达，但其他成员的表达却受到很大限制。 HRP-1和-4仅在睾丸中表达，HRP-3仅在神经系统中表达。在这里，我们通过免疫组织化学研究了成年小鼠中枢神经系统中HDGF，HRP-2和HRP-3在细胞水平上的表达。此外，我们对各种大脑区域以及神经元和神经胶质细胞培养物进行了蛋白质印迹分析。结果HDGF在所有测试的脑区域中均等地表达，在黑质中表达最低。 HRP-2在丘脑，额叶和顶叶皮层，神经垂体和小脑中强烈表达，HRP-3在嗅球，梨状皮层和杏仁核复合体中表达。发现HDGF和HRP-2由神经元，星形胶质细胞和少突胶质细胞表达。相反，除了脑干中少突胶质细胞中非常弱的表达外，HRP-3在成人神经系统中的强表达只限于神经元。尽管大多数神经元为HRP-3阳性，但某些小脑颗粒细胞为阴性。结论HDGF和HRP-2在神经胶质和神经元中的共表达以及在许多神经元中所有三种蛋白的共表达表明，HDGF蛋白家族成员在中枢神经系统细胞中的功能不同，可能包括增殖和细胞增殖。生存。另外，HRP-3的限制性表达表明该家族成员对于神经元细胞的特殊功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号