• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Microbiology Research Journal >Plasmid-Mediated Quinolone Resistance Genes qnrA, qnrB, qnrC, qnrD, qnrS and aac (6')-Ib in Pseudomonas aeruginosa and Acinetobacter baumannii
【24h】

Plasmid-Mediated Quinolone Resistance Genes qnrA, qnrB, qnrC, qnrD, qnrS and aac (6')-Ib in Pseudomonas aeruginosa and Acinetobacter baumannii

机译：铜绿假单胞菌和鲍曼不动杆菌中质粒介导的喹诺酮抗性基因qnrA，qnrB，qnrC，qnrD，qnrS和aac（6'）-Ib

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: The emergence and spread of antimicrobial-resistant bacterial isolates is of great concern, especially to commonly used antimicrobials as Quinolones.Aim of the Study: Estimation of the prevalence of quinolone-resistant P. aeruginosa and A. baumannii in various clinical specimens, detection and characterization of the pattern of plasmid-mediated quinolone resistance genes present.Materials and Methods: Bacterial identification of P. aeruginosa (92) and A. baumannii (69) strains was performed using standard laboratory methods. Antimicrobial susceptibility to other antibiotic was determined by the standard Kirby- Bauer disk diffusion method. All collected quinolones resistant isolates (100) were identified and further subjected to conventional PCR for detection of quinolones resistant genes; namely qnrA, qnrB, qnrC, qnrD, qnrS and aac (6’)-Ib, using specific primers. Sequencing were done for all PMQR positive strains using A BigDye Terminator v3.1 cycle sequencing kit. Results: PCR revealed 46% of examined quinolone-resistant isolates have one or more of PMQR genes, A. baumannii 28/58 (48.3%) and P. aeruginosa 18/42 (42.9%). The predominant gene detected was aac (6’)-Ib either alone or in combination with other genes. There was significant isolates harboring qnrS gene among P. aeuroginsa which was mainly isolated from outpatients. Sequencing of the PMQR positive strains has confirmed the results of PCR without detection of any new varients or disconcordant strains. Conclusions: The prevalence rate of quinolone-resistant P. aeruginosa and A. baumannii harboring PMQR genes is high in our hospitals. The acc(6)-1b gene is the PMQR predominant gene coding for quinolone resistance.
机译：背景：耐药菌分离株的出现和传播引起了人们的极大关注，尤其是对于常用的抗菌药物喹诺酮类。研究目的：估计各种临床标本中对喹诺酮耐药的铜绿假单胞菌和鲍曼不动杆菌的患病率，材料和方法：使用标准实验室方法对铜绿假单胞菌（92）和鲍曼不动杆菌（69）菌株进行细菌鉴定。通过标准Kirby-Bauer圆盘扩散法确定对其他抗生素的抗菌敏感性。鉴定出所有收集的喹诺酮抗性分离株（100），并进一步进行常规PCR检测喹诺酮类抗性基因。使用特定引物，即qnrA，qnrB，qnrC，qnrD，qnrS和aac（6’）-Ib。使用BigDye Terminator v3.1循环测序试剂盒对所有PMQR阳性菌株进行测序。结果：PCR结果显示，有46％的耐喹诺酮分离株具有一个或多个PMQR基因，鲍曼不动杆菌28/58（48.3％）和铜绿假单胞菌18/42（42.9％）。检测到的主要基因是单独或与其他基因结合的aac（6'）-Ib。在嗜水气单胞菌中有大量带有qnrS基因的分离株，主要从门诊病人身上分离出来。 PMQR阳性菌株的测序已证实PCR的结果，而未检测到任何新的变体或不一致的菌株。结论：在我院，对喹诺酮类耐药的铜绿假单胞菌和鲍曼不动杆菌带有PMQR基因的患病率较高。 acc（6）-1b基因是PMQR编码喹诺酮耐药性的主要基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号