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首页> 外文期刊>BMC Plant Biology >Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum
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Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum

机译：谷物半胱氨酸蛋白酶抑制剂对马铃薯马铃薯马铃薯转基因品系的有益“意外影响”

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Background Studies reported unintended pleiotropic effects for a number of pesticidal proteins ectopically expressed in transgenic crops, but the nature and significance of such effects in planta remain poorly understood. Here we assessed the effects of corn cystatin II (CCII), a potent inhibitor of C1A cysteine (Cys) proteases considered for insect and pathogen control, on the leaf proteome and pathogen resistance status of potato lines constitutively expressing this protein. Results The leaf proteome of lines accumulating CCII at different levels was resolved by 2-dimensional gel electrophoresis and compared with the leaf proteome of a control (parental) line. Out of ca. 700 proteins monitored on 2-D gels, 23 were significantly up- or downregulated in CCII-expressing leaves, including 14 proteins detected de novo or up-regulated by more than five-fold compared to the control. Most up-regulated proteins were abiotic or biotic stress-responsive proteins, including different secretory peroxidases, wound inducible protease inhibitors and pathogenesis-related proteins. Accordingly, infection of leaf tissues by the fungal necrotroph Botryris cinerea was prevented in CCII-expressing plants, despite a null impact of CCII on growth of this pathogen and the absence of extracellular Cys protease targets for the inhibitor. Conclusions These data point to the onset of pleiotropic effects altering the leaf proteome in transgenic plants expressing recombinant protease inhibitors. They also show the potential of these proteins as ectopic modulators of stress responses in planta, useful to engineer biotic or abiotic stress tolerance in crop plants of economic significance.
机译：背景研究报道了在转基因作物中异位表达的多种杀虫蛋白具有意想不到的多效作用，但是这种作用在植物中的性质和重要性仍然知之甚少。在这里，我们评估了玉米胱抑素II（CCII）（一种用于昆虫和病原体控制的有效C1A半胱氨酸（Cys）蛋白酶抑制剂）对组成型表达该蛋白的马铃薯品系的叶片蛋白质组和病原体抗性状态的影响。结果通过二维凝胶电泳解析了CCII在不同水平上积累的品系的叶片蛋白质组，并与对照（亲本）品系的叶片蛋白质组进行了比较。超出在2-D凝胶上监测的700种蛋白质中，有23种在表达CCII的叶片中显着上调或下调，其中包括从头检测到的14种蛋白质或与对照相比上调五倍的蛋白质。大多数上调的蛋白是非生物或生物应激反应蛋白，包括不同的分泌性过氧化物酶，伤口诱导型蛋白酶抑制剂和发病相关蛋白。因此，尽管CCII对该病原体的生长没有影响，并且不存在抑制剂的细胞外Cys蛋白酶靶标，但是在表达CCII的植物中真菌的坏死性灰葡萄孢的灰霉病对叶片组织的感染被阻止。结论这些数据表明，在表达重组蛋白酶抑制剂的转基因植物中，多效性效应改变了叶片蛋白质组。他们还显示了这些蛋白作为植物体内压力反应的异位调节剂的潜力，可用于设计具有重要经济意义的作物植物的生物或非生物胁迫耐受性。

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