...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Plant Biology >Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata , enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes
【24h】

Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata , enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes

机译：PtrABF基因的过量表达是一种分离自三叶草的bZIP转录因子，可通过清除ROS和调节压力响应基因的表达来增强烟草的脱水和干旱耐受性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Drought is one of the major abiotic stresses affecting plant growth, development and crop productivity. ABA responsive element binding factor (ABF) plays an important role in stress responses via regulating the expression of stress-responsive genes. Results In this study, a gene coding for ABF ( PtrABF ) was isolated from Poncirus trifoliata (L.) Raf. PtrABF had a complete open reading frame of 1347 bp, encoding a 448 amino acid peptide, and shared high sequence identities with ABFs from other plants. PtrABF was subcellularly targeted to the nucleus, exhibited transactivation activity in yeast cell and could bind to ABRE, supporting its role as a transcription factor. Expression levels of PtrABF were induced by treatments with dehydration, low temperature and ABA. Ectopic expression of PtrABF under the control of a CaMV 35S promoter in transgenic tobacco plants enhanced tolerance to both dehydration and drought. Under dehydration and drought conditions, the transgenic plants accumulated lower levels of reactive oxygen species compared with wild type, accompanied by higher activities and expression levels of three antioxidant enzymes. In addition, steady-state mRNA levels of nine stress-responsive genes coding for either functional or regulatory proteins were induced to higher levels in the transgenic lines with or without drought stress. Conclusions PtrABF is a bZIP transcription factor and functions in positive modulation of drought stress tolerance. It may be an important candidate gene for molecular breeding of drought- tolerant plants.
机译：背景技术干旱是影响植物生长，发育和作物生产力的主要非生物胁迫之一。 ABA响应元件结合因子（ABF）通过调节应激反应基因的表达在应激反应中起重要作用。结果在这项研究中，从三叶草（R.）Raf中分离到一个编码ABF（PtrABF）的基因。 PtrABF具有1347 bp的完整开放阅读框，编码448个氨基酸的肽，并与其他植物的ABF具有高度的序列同一性。 PtrABF被亚细胞靶向核，在酵母细胞中表现出反式激活活性，并可以与ABRE结合，支持其作为转录因子的作用。通过脱水，低温和ABA处理诱导PtrABF的表达水平。在CaMV 35S启动子的控制下，PtrABF在转基因烟草植物中的异位表达增强了对脱水和干旱的耐受性。在干旱和干旱条件下，与野生型相比，转基因植物积累的活性氧水平较低，同时伴随着较高的活性和三种抗氧化酶的表达水平。另外，在有干旱胁迫或没有干旱胁迫的转基因品系中，编码功能或调节蛋白的九个胁迫响应基因的稳态mRNA水平被诱导到更高的水平。结论PtrABF是bZIP转录因子，在干旱胁迫耐受性的正调控中起作用。它可能是耐旱植物分子育种的重要候选基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号