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首页> 外文期刊>BMC Plant Biology >Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues
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Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues

机译：磷酸甘露糖异位酶（PMM）基因的分子分析揭示了小麦中不同种类的小麦中独特的PMM复制事件以及面包小麦组织中的主要PMM同工酶

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Background Phosphomannomutase (PMM) is an essential enzyme in eukaryotes. However, little is known about PMM gene and function in crop plants. Here, we report molecular evolutionary and biochemical analysis of PMM genes in bread wheat and related Triticeae species. Results Two sets of homoeologous PMM genes ( TaPMM-1 and 2 ) were found in bread wheat, and two corresponding PMM genes were identified in the diploid progenitors of bread wheat and many other diploid Triticeae species. The duplication event yielding PMM-1 and 2 occurred before the radiation of diploid Triticeae genomes. The PMM gene family in wheat and relatives may evolve largely under purifying selection. Among the six TaPMM genes, the transcript levels of PMM-1 members were comparatively high and their recombinant proteins were all enzymatically active. However, PMM-2 homoeologs exhibited lower transcript levels, two of which were also inactive. TaPMM-A1, B1 and D1 were probably the main active isozymes in bread wheat tissues. The three isozymes differed from their counterparts in barley and Brachypodium distachyon in being more tolerant to elevated test temperatures. Conclusion Our work identified the genes encoding PMM isozymes in bread wheat and relatives, uncovered a unique PMM duplication event in diverse Triticeae species, and revealed the main active PMM isozymes in bread wheat tissues. The knowledge obtained here improves the understanding of PMM evolution in eukaryotic organisms, and may facilitate further investigations of PMM function in the temperature adaptability of bread wheat.
机译：背景技术磷酸甘露糖突变酶（PMM）是真核生物中必不可少的酶。然而，关于农作物中PMM基因和功能的了解甚少。在这里，我们报告面包小麦和相关小麦品种中PMM基因的分子进化和生化分析。结果在面包小麦中发现了两组同源的PMM基因（TaPMM-1和2），并在面包小麦和许多其他二倍体小麦的二倍体祖先中鉴定出两个相应的PMM基因。产生PMM-1和2的复制事件发生在辐射二倍体小麦基因组之前。在纯化选择下，​​小麦及其亲属的PMM基因家族可能会在很大程度上进化。在六个TaPMM基因中，PMM-1成员的转录水平较高，并且它们的重组蛋白均具有酶促活性。然而，PMM-2同系物表现出较低的转录水平，其中两个也没有活性。 TaPMM-A1，B1和D1可能是面包小麦组织中的主要活性同工酶。三种同工酶与大麦和短枝曲霉的同工酶相比，在更高的测试温度下更耐。结论我们的工作鉴定了面包小麦及其亲属中编码PMM同工酶的基因，揭示了在多种小麦属物种中独特的PMM复制事件，并揭示了面包小麦组织中的主要活性PMM同工酶。此处获得的知识增进了对真核生物中PMM进化的理解，并可能有助于进一步研究PMM在面包小麦温度适应性中的功能。

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