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首页> 外文期刊>BMC Plant Biology >A stress inducible SUMO conjugating enzyme gene (SaSce9) from a grass halophyte Spartina alterniflora enhances salinity and drought stress tolerance in Arabidopsis
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A stress inducible SUMO conjugating enzyme gene (SaSce9) from a grass halophyte Spartina alterniflora enhances salinity and drought stress tolerance in Arabidopsis

机译：盐生植物互花米草（Spartina alterniflora）的胁迫诱导SUMO结合酶基因（SaSce9）增强了拟南芥的盐度和干旱胁迫耐受性

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Background SUMO (Small Ubiquitin related Modifier) conjugation is a post translational regulatory process found in all eukaryotes, mediated by SUMO activating enzyme, SUMO conjugating enzyme, and SUMO ligase for the attachment of SUMO to its target protein. Although the mechanism for regulation of SUMO conjugation pathway genes under abiotic stress has been studied to certain extent, the role of SUMO conjugating enzyme in improving abiotic stress tolerance to plant is largely unexplored. Here, we have characterized a SUMO conjugating enzyme gene ‘SaSce9’ from a halophytic grass Spartina alterniflora and investigated its role in imparting abiotic stress tolerance. Results SaSce9 gene encodes for a polypeptide of 162 amino acids with a molecular weight of ~18 kD and isoelectric point 8.43. Amino acid sequence comparisons of SaSce9 with its orthologs from other plant species showed high degree (~85-93%) of structural conservation among each other. Complementation analysis using yeast SCE mutant, Ubc9, revealed functional conservation of SaSce9 between yeast and S. alterniflora. SaSce9 transcript was inducible by salinity, drought, cold, and exogenously supplied ABA both in leaves and roots of S. alterniflora. Constitutive overexpression of SaSce9 in Arabidopsis through Agrobacterium mediated transformation improved salinity and drought tolerance of Arabidopsis. SaSce9 overexpressing Arabidopsis plants retained more chlorophyll and proline both under salinity and drought stress. SaSce9 transgenic plants accumulated lower levels of reactive oxygen under salinity stress. Expression analysis of stress responsive genes in SaSce9 Arabidopsis plants revealed the increased expression of antioxidant genes, AtSOD and AtCAT, ion antiporter genes, AtNHX1 and AtSOS1, a gene involved in proline biosynthesis, AtP5CS, and a gene involved in ABA dependent signaling pathway, AtRD22. Conclusions These results highlight the prospect of improving abiotic stress tolerance in plants through genetic engineering of the sumoylation pathway. The study provides evidence that the overexpression of SaSce9 in plant can improve salinity and drought stress tolerance by protecting the plant through scavenging of ROS, accumulation of an osmolyte, proline, and expression of stress responsive genes. In addition, this study demonstrates the potential of the halophyte grass S. alterniflora as a reservoir of abiotic stress related genes for crop improvement.
机译：背景SUMO（小泛素相关修饰剂）偶联是在所有真核生物中发现的翻译后调控过程，由SUMO活化酶，SUMO偶联酶和SUMO连接酶介导，以将SUMO附着于其靶蛋白。尽管已经在一定程度上研究了在非生物胁迫下调节SUMO缀合途径基因的机制，但是在很大程度上尚未探索到SUMO缀合酶在改善植物的非生物胁迫耐受性中的作用。在这里，我们表征了盐生植物草互花米草的SUMO偶联酶基因'SaSce9'，并研究了其在赋予非生物胁迫耐受性中的作用。结果SaSce9基因编码一个162个氨基酸的多肽，分子量约为18 kD，等电点为8.43。 SaSce9与其他植物直系同源物的氨基酸序列比较表明，彼此之间的结构保守性很高（〜85-93％）。使用酵母SCE突变体Ubc9进行的互补分析揭示了SaSce9在酵母和互生链球菌之间的功能保守性。盐度，干旱，寒冷和互生链球菌根和叶中的ABA均可诱导SaSce9转录本。通过农杆菌介导的转化在拟南芥中组成型SaSce9的过表达改善了拟南芥的盐度和干旱耐受性。在盐分和干旱胁迫下，过表达SaSce9的拟南芥植物保留了更多的叶绿素和脯氨酸。 SaSce9转基因植物在盐胁迫下积累的活性氧水平较低。 SaSce9拟南芥植物中应激反应基因的表达分析表明，抗氧化基因，AtSOD和AtCAT，离子反转运蛋白基因，AtNHX1和AtSOS1（脯氨酸生物合成所涉及的基因）AtP5CS以及与ABA依赖的信号传导途径有关的基因AtRD22的表达增加。 。结论这些结果突出了通过sumoylation途径的基因工程改善植物对非生物胁迫的耐受性的前景。这项研究提供了证据，表明SaSce9在植物中的过表达可以通过清除ROS，渗透压，脯氨酸的积累和胁迫响应基因的表达来保护植物，从而提高盐度和干旱胁迫耐受性。此外，这项研究证明了盐生植物互花米草作为非生物胁迫相关基因的贮藏库对作物改良的潜力。

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