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首页> 外文期刊>BMC Plant Biology >Light-induced morphological alteration in anthocyanin-accumulating vacuoles of maize cells
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Light-induced morphological alteration in anthocyanin-accumulating vacuoles of maize cells

机译：光诱导玉米细胞花青素富集液泡中的形态变化

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Background Plant pigmentation is affected by a variety of factors. Light, an important plant developmental signal, influences the accumulation of anthocyanins primarily through the activation of the transcription factors that regulate the flavonoid biosynthetic pathway. In this study, we utilized maize Black Mexican Sweet (BMS) cells expressing the R and C1 regulators of anthocyanin biosynthesis from a light-insensitive promoter as a means to investigate the existence of additional levels of control of pigmentation by light. Results BMS cells expressing the R and C1 regulators from the CaMV 35S constitutive promoter accumulate anthocyanins when grown in complete darkness, suggesting that the transcription factors R and C1 are sufficient for the transcription of the genes corresponding to the structural enzymes of the pathway, with no requirement for additional light-induced regulators. Interestingly, light induces a "darkening" in the color of the purple anthocyanin pigmentation of transgenic BMS cells expressing R and C1. This change in the pigment hue is not associated with a variation in the levels or types of anthocyanins present, or with an alteration of the transcript levels of several flavonoid biosynthetic genes. However, cytological observations show that light drives unexpected changes in the morphology and distribution of the anthocyanins-containing vacuolar compartments. Conclusion By uncoupling the effect of light on anthocyanin accumulation, we have found light to induce the fusion of anthocyanin-containing vacuoles, the coalescence of anthocyanic vacuolar inclusion (AVI)-like structures contained, and the spread of anthocyanins from the inclusions into the vacuolar sap. Similar light-induced alterations in vacuolar morphology are also evident in the epidermal cells of maize floral whorls accumulating anthocyanins. Our findings suggest a novel mechanism for the action of light on the vacuolar storage of anthocyanin.
机译：背景植物色素沉着受多种因素影响。光是一种重要的植物发育信号，主要通过调节类黄酮生物合成途径的转录因子的激活来影响花色苷的积累。在这项研究中，我们利用玉米黑墨西哥甜（BMS）细胞来表达对光不敏感的启动子的花色苷生物合成的R和C1调节剂，作为研究通过光控制色素沉着的附加水平的方法。结果从CaMV 35S组成型启动子表达R和C1调节剂的BMS细胞在完全黑暗中生长时会积累花青素，这表明转录因子R和C1足以转录与该途径的结构酶相对应的基因，而没有需要额外的光感应调节器。有趣的是，光诱导表达R和C1的BMS细胞的紫色花色苷色素沉着。色素色调的这种变化与花色苷水平或类型的变化或几种类黄酮生物合成基因的转录水平的变化均无关。然而，细胞学观察表明，光驱使含花青素的液泡区室的形态和分布发生意想不到的变化。结论通过解开光对花色苷积累的影响，我们发现光可诱导含花色苷的液泡融合，所含花色液泡内含物（AVI）样结构的聚结，以及花色苷从内含物扩散到液泡中树液。在积累花色苷的玉米花轮的表皮细胞中，液泡形态的类似光诱导变化也很明显。我们的发现提示光对花青素的液泡储存的作用的新机制。

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