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首页> 外文期刊>BMC Plant Biology >Design and fabrication of adjustable red-green-blue LED light arrays for plant research
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Design and fabrication of adjustable red-green-blue LED light arrays for plant research

机译:用于植物研究的可调节红绿蓝LED灯阵列的设计与制造

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Background Although specific light attributes, such as color and fluence rate, influence plant growth and development, researchers generally cannot control the fine spectral conditions of artificial plant-growth environments. Plant growth chambers are typically outfitted with fluorescent and/or incandescent fixtures that provide a general spectrum that is accommodating to the human eye and not necessarily supportive to plant development. Many studies over the last several decades, primarily in Arabidopsis thaliana , have clearly shown that variation in light quantity, quality and photoperiod can be manipulated to affect growth and control developmental transitions. Light emitting diodes (LEDs) has been used for decades to test plant responses to narrow-bandwidth light. LEDs are particularly well suited for plant growth chambers, as they have an extraordinary life (about 100,000 hours), require little maintenance, and use negligible energy. These factors render LED-based light strategies particularly appropriate for space-biology as well as terrestrial applications. However, there is a need for a versatile and inexpensive LED array platform where individual wavebands can be specifically tuned to produce a series of light combinations consisting of various quantities and qualities of individual wavelengths. Two plans are presented in this report. Results In this technical report we describe the practical construction of tunable red-green-blue LED arrays to support research in plant growth and development. Two light fixture designs and corresponding circuitry are presented. The first is well suited for a laboratory environment for use in a finite area with small plants, such as Arabidopsis. The second is expandable and appropriate for growth chambers. The application of these arrays to early plant developmental studies has been validated with assays of hypocotyl growth inhibition/promotion and phototropic curvature in Arabidopsis seedlings. Conclusion The presentation of these proven plans for LED array construction allows the teacher, researcher or electronics aficionado a means to inexpensively build efficient, adjustable lighting modules for plant research. These simple and effective designs permit the construction of useful tools by programs short on electronics expertise. These arrays represent a means to modulate precise quality and quantity in experimental settings to test the effect of specific light combinations in regulating plant growth, development and plant-product yield.
机译:背景技术尽管特定的光照属性(例如颜色和通量率)会影响植物的生长和发育,但研究人员通常无法控制人造植物生长环境的精细光谱条件。植物生长室通常配备有荧光灯和/或白炽灯灯具,这些灯具可以提供适合人眼的总光谱,而不一定支持植物的生长。在过去的几十年中,许多研究(主要是在拟南芥中)清楚地表明,可以控制光量,质量和光周期的变化以影响生长并控制发育过渡。数十年来,发光二极管(LED)已用于测试工厂对窄带宽光的响应。 LED具有非常长的使用寿命(大约100,000小时),几乎不需要维护并且使用的能量很少,因此特别适合植物生长室。这些因素使得基于LED的照明策略特别适合于空间生物学以及地面应用。然而,需要一种通用且廉价的LED阵列平台,其中可以具体地调节各个波段以产生一系列由各种数量和质量的各个波长组成的光组合。本报告提出了两个计划。结果在本技术报告中,我们描述了可调谐红绿蓝LED阵列的实际构造,以支持植物生长和发育的研究。提出了两种灯具设计和相应的电路。第一种非常适合在有限区域内使用小型植物(如拟南芥)的实验室环境。第二个是可扩展的,适用于生长室。这些阵列在早期植物发育研究中的应用已通过测定拟南芥幼苗中下胚轴生长抑制/促进和向光性弯曲的方法得到验证。结论通过这些行之有效的LED阵列设计方案的介绍,教师,研究人员或电子爱好者可以廉价地构建用于工厂研究的高效可调节照明模块。这些简单有效的设计允许通过缺少电子专业知识的程序来构建有用的工具。这些阵列代表一种在实验设置中调节精确质量和数量的方法,以测试特定光组合在调节植物生长,发育和植物产品产量中的作用。

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