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首页> 外文期刊>BMC Plant Biology >Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus
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Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus

机译：鹰嘴豆根EST的开发及其在Cicer属多样性分析中的应用

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Background Chickpea is a major crop in many drier regions of the world where it is an important protein-rich food and an increasingly valuable traded commodity. The wild annual Cicer species are known to possess unique sources of resistance to pests and diseases, and tolerance to environmental stresses. However, there has been limited utilization of these wild species by chickpea breeding programs due to interspecific crossing barriers and deleterious linkage drag. Molecular genetic diversity analysis may help predict which accessions are most likely to produce fertile progeny when crossed with chickpea cultivars. While, trait-markers may provide an effective tool for breaking linkage drag. Although SSR markers are the assay of choice for marker-assisted selection of specific traits in conventional breeding populations, they may not provide reliable estimates of interspecific diversity, and may lose selective power in backcross programs based on interspecific introgressions. Thus, we have pursued the development of gene-based markers to resolve these problems and to provide candidate gene markers for QTL mapping of important agronomic traits. Results An EST library was constructed after subtractive suppressive hybridization (SSH) of root tissue from two very closely related chickpea genotypes ( Cicer arietinum ). A total of 106 EST-based markers were designed from 477 sequences with functional annotations and these were tested on C. arietinum . Forty-four EST markers were polymorphic when screened across nine Cicer species (including the cultigen). Parsimony and PCoA analysis of the resultant EST-marker dataset indicated that most accessions cluster in accordance with the previously defined classification of primary ( C. arietinum , C. echinospermum and C. reticulatum ), secondary ( C. pinnatifidum , C. bijugum and C. judaicum ), and tertiary ( C. yamashitae , C. chrossanicum and C. cuneatum ) gene-pools. A large proportion of EST alleles (45%) were only present in one or two of the accessions tested whilst the others were represented in up to twelve of the accessions tested. Conclusion Gene-based markers have proven to be effective tools for diversity analysis in Cicer and EST diversity analysis may be useful in identifying promising candidates for interspecific hybridization programs. The EST markers generated in this study have detected high levels of polymorphism amongst both common and rare alleles. This suggests that they would be useful for allele-mining of germplasm collections for identification of candidate accessions in the search for new sources of resistance to pests / diseases, and tolerance to abiotic stresses.
机译：背景鹰嘴豆是世界上许多干旱地区的主要农作物，在这里它是一种重要的富含蛋白质的食品，也是一种越来越有价值的商品。众所周知，野生一年生的Cicer物种具有独特的抗病虫害能力和对环境压力的耐受力。但是，由于种间杂交障碍和有害的连接阻力，鹰嘴豆育种程序对这些野生物种的利用受到限制。分子遗传多样性分析可以帮助预测哪些品种与鹰嘴豆品种杂交时最有可能产生可育后代。同时，特征标记可能会提供一种有效的工具来打破连锁阻力。尽管SSR标记是常规育种种群中特定性状的标记辅助选择的选择分析方法，但它们可能无法提供可靠的种间多样性估计，并且可能会在基于种间渗入的回交程序中丧失选择力。因此，我们已经着手开发基于基因的标记来解决这些问题，并为重要的农艺性状的QTL作图提供候选的基因标记。结果从两个密切相关的鹰嘴豆基因型（Cicer arietinum）的根组织进行消减抑制杂交（SSH）后，构建了一个EST文库。从477个具有功能注释的序列设计了总共106个基于EST的标记，并在A. arietinum上进行了测试。当在9种Cicer物种（包括栽培种）中进行筛选时，有44种EST标记具有多态性。对得到的EST标记数据集的简约和PCoA分析表明，大多数登录品都按照先前定义的初级（C. arietinum，C。echinospermum和C. reticulatum），次生（C. pinnatifidum，C。bijugum和C）的分类进行聚类。菊科植物（Judaicum）和三级植物（C. yamashitae，C。chrossanicum和C. cuneatum）基因库。大部分EST等位基因（45％）仅出现在一个或两个测试的种质中，而其他则最多出现在测试的12个种质中。结论基于基因的标记物已被证明是在Cicer中进行多样性分析的有效工具，而EST多样性分析对于确定种间杂交计划的有希望的候选者可能有用。这项研究中产生的EST标记已在常见和罕见等位基因之间检测到高水平的多态性。这表明它们将有助于种质资源的等位基因挖掘，从而在寻找新的抗病虫害和抗非生物胁迫的来源时，鉴定候选种。

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