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首页> 外文期刊>BMC Plant Biology >Transcriptomic changes reveal gene networks responding to the overexpression of a blueberry DWARF AND DELAYED FLOWERING 1 gene in transgenic blueberry plants
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Transcriptomic changes reveal gene networks responding to the overexpression of a blueberry DWARF AND DELAYED FLOWERING 1 gene in transgenic blueberry plants

机译：转录组学变化揭示了基因网络对转基因蓝莓植物中蓝莓DWARF和延迟开花1基因的过度表达有反应

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Background Constitutive expression of the CBF/DREB1 for increasing freezing tolerance in woody plants is often associated with other phenotypic changes including dwarf plant and delayed flowering. These phenotypic changes have been observed when Arabidopsis DWARF AND DELAYED FLOWERING 1 ( DDF1 ) was overexpressed in A. thaliana plants. To date, the DDF1 orthologues have not been studied in woody plants. The aim of this study is to investigate transcriptomic responses to the overexpression of blueberry ( Vaccinium corymbosum ) DDF1 (herein, VcDDF1 -OX). Results The VcDDF1 -OX resulted in enhanced freezing tolerance in tetraploid blueberry plants and did not result in significant changes in plant size, chilling requirement, and flowering time. Comparative transcriptome analysis of transgenic ‘Legacy-VcDDF1-OX’ plants containing an overexpressed VcDDF1 with non-transgenic highbush blueberry ‘Legacy’ plants revealed the VcDDF1- OX derived differentially expressed (DE) genes and transcripts in the pathways of cold-response, plant flowering, DELLA proteins, and plant phytohormones. The increase in freezing tolerance was associated to the expression of cold-regulated genes (CORs) and the ethylene pathway genes. The unchanged plant size, dormancy and flowering were due to the minimal effect of the VcDDF1- OX on the expression of DELLA proteins, flowering pathway genes, and the other phytohormone genes related to plant growth and development. The DE genes in auxin and cytokinin pathways suggest that the VcDDF1- OX has also altered plant tolerance to drought and high salinity. Conclusion A DDF1 orthologue in blueberry functioned differently from the DDF1 reported in Arabidopsis . The overexpression of VcDDF1 or its orthologues is a new approach to increase freezing tolerance of deciduous woody plant species with no obvious effect on plant size and plant flowering time.
机译：背景CBF / DREB1的组成型表达可提高木本植物的耐冻性，通常与其他表型变化有关，包括矮化植物和延迟开花。当拟南芥DWARF和延迟开花1（DDF1）在拟南芥植物中过表达时，已经观察到这些表型变化。迄今为止，尚未在木本植物中研究DDF1直向同源物。这项研究的目的是调查对蓝莓（Vaccinium corymbosum）DDF1（此处为VcDDF1-OX）过表达的转录组学反应。结果VcDDF1-OX增强了四倍体蓝莓植株的抗冻性，并且未导致植株大小，冷藏需求和开花时间的显着变化。对含有过量表达的VcDDF1的转基因'Legacy-VcDDF1-OX'植物与非转基因高灌木蓝莓'Legacy'植物进行的转录组比较分析显示，VcDDF1- OX源自差异表达（DE）基因和转录本，其反应方式为植物开花，DELLA蛋白和植物激素。冷冻耐受性的增加与冷调节基因（CORs）和乙烯途径基因的表达有关。不变的植物大小，休眠和开花是由于VcDDF1-OX对DELLA蛋白，开花途径基因和其他与植物生长发育相关的植物激素基因表达的影响最小。生长素和细胞分裂素途径中的DE基因表明，VcDDF1-OX还改变了植物对干旱和高盐分的耐受性。结论蓝莓中DDF1直向同源物的功能与拟南芥中报道的DDF1不同。 VcDDF1或其直系同源物的过表达是增加落叶木本植物抗冻性的新方法，对植物大小和开花时间没有明显影响。

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