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首页> 外文期刊>BMC Plant Biology >Chemical hybridizing agent SQ-1-induced male sterility in Triticum aestivum L.: a comparative analysis of the anther proteome
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Chemical hybridizing agent SQ-1-induced male sterility in Triticum aestivum L.: a comparative analysis of the anther proteome

机译：化学杂交剂SQ-1诱导的普通小麦雄性不育：花药蛋白质组的比较分析

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Heterosis is widely used to increase the yield of many crops. However, as wheat is a self-pollinating crop, hybrid breeding is not so successful in this organism. Even though male sterility induced by chemical hybridizing agents is an important aspect of crossbreeding, the mechanisms by which these agents induce male sterility in wheat is not well understood. We performed proteomic analyses using the wheat Triticum aestivum L.to identify those proteins involved in physiological male sterility (PHYMS) induced by the chemical hybridizing agent CHA SQ-1. A total of 103 differentially expressed proteins were found by 2D–PAGE and subsequently identified by MALDI-TOF/TOF MS/MS. In general, these proteins had obvious functional tendencies implicated in carbohydrate metabolism, oxidative stress and resistance, protein metabolism, photosynthesis, and cytoskeleton and cell structure. In combination with phenotypic, tissue section, and bioinformatics analyses, the identified differentially expressed proteins revealed a complex network behind the regulation of PHYMS and pollen development. Accordingly, we constructed a protein network of male sterility in wheat, drawing relationships between the 103 differentially expressed proteins and their annotated biological pathways. To further validate our proposed protein network, we determined relevant physiological values and performed real-time PCR assays. Our proteomics based approach has enabled us to identify certain tendencies in PHYMS anthers. Anomalies in carbohydrate metabolism and oxidative stress, together with premature tapetum degradation, may be the cause behind carbohydrate starvation and male sterility in CHA SQ-1 treated plants. Here, we provide important insight into the mechanisms underlying CHA SQ-1-induced male sterility. Our findings have practical implications for the application of hybrid breeding in wheat.
机译：杂种优势被广泛用于增加许多农作物的产量。但是，由于小麦是自花授粉作物，因此在这种生物中杂交育种并不那么成功。尽管由化学杂交剂诱导的雄性不育是杂交的重要方面，但这些试剂诱导小麦中雄性不育的机理仍未得到很好的理解。我们使用小麦小麦进行了蛋白质组学分析，以鉴定与化学杂化剂CHA SQ-1诱导的生理雄性不育（PHYMS）有关的蛋白质。通过2D-PAGE共找到103种差异表达的蛋白质，然后通过MALDI-TOF / TOF MS / MS进行鉴定。通常，这些蛋白质具有明显的功能趋势，涉及碳水化合物代谢，氧化应激和抗性，蛋白质代谢，光合作用以及细胞骨架和细胞结构。结合表型，组织切片和生物信息学分析，鉴定出的差异表达蛋白揭示了PHYMS和花粉发育调控背后的复杂网络。因此，我们构建了小麦中雄性不育的蛋白质网络，绘制了103种差异表达的蛋白质与其注释的生物途径之间的关系。为了进一步验证我们提出的蛋白质网络，我们确定了相关的生理值并进行了实时PCR分析。我们基于蛋白质组学的方法使我们能够识别PHYMS花药中的某些趋势。在CHA SQ-1处理过的植物中，碳水化合物代谢异常和氧化应激异常，以及过早的绒毡层降解可能是导致碳水化合物饥饿和雄性不育的原因。在这里，我们提供了对CHA SQ-1诱导男性不育的潜在机制的重要见解。我们的发现对小麦杂交育种的应用具有实际意义。

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