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首页> 外文期刊>Brazilian Journal of Microbiology >Cellulase activities during decomposition of a submerged aquatic macrophyte (Utricularia breviscapa): a microcosm assay
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Cellulase activities during decomposition of a submerged aquatic macrophyte (Utricularia breviscapa): a microcosm assay

机译：淹没的水生植物（Utricularia breviscapa）分解过程中的纤维素酶活性：微观分析

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摘要

Decomposition of lignocellulosic detritus at enzymatic level is the rate-limiting step during aquatic macrophyte decomposition. Assays were carried out to evaluate cellulase activity during decomposition of Utricularia breviscapa; the assays included the following C-sources: leachate, lignocellulosic matrix and integral detritus. The incubations comprised U. breviscapa C-sources added to óleo lagoon (21o 36'S and 47o 49'W) water maintained in the dark under anaerobic condition at 15oC, 20oC, 25oC and 30oC. Cellulase activity were monitored during 180 days, with the activity showing a time evolution that depended on the temperature and type of detritus: (i) cellulase activity tended to occur earlier with increasing temperatures, but it was less intense than at lower temperatures, (ii) no activity was observed in incubations with leachate, (iii) cellulase activity was observed in the incubation with integral detritus and lignocellulosic matrix and (iv) higher cellulase activity was observed in the incubations with integral detritus at 15oC (P < 0.05).
机译：木质纤维素碎屑在酶促水平上的分解是水生植物分解过程中的限速步骤。进行了测定，以评价短壁乌头菌分解过程中的纤维素酶活性。分析包括以下碳源：渗滤液，木质纤维素基质和碎屑。孵化包括将灯盏花U碳源添加到在15oC，20oC，25oC和30oC的厌氧条件下保持在黑暗中的óleo泻湖（21o 36'S和47o 49'W）水中。在180天内对纤维素酶的活性进行了监测，其活性随时间的变化取决于碎屑的温度和类型：（i）纤维素酶的活性往往随温度升高而提前发生，但强度却低于低温时的强度（ii ）在浸出液培养中未观察到活性，（iii）在整合碎屑和木质纤维素基质中观察到纤维素酶活性，以及​​（iv）在15°C整合碎屑中观察到更高的纤维素酶活性（P <0.05）。

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