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首页> 外文期刊>Brazilian Archives of Biology and Technology >Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
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Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces

机译：分离自人类粪便的芒氏肠球菌产生的肠毒素

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摘要

The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121oC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite and a molecular weight similar to many purified enterocins.
机译：这项研究的目的是鉴定70株芒氏肠球菌产生的细菌素。四种菌株对无毒李斯特菌，单核细胞增生李斯特菌，植物乳杆菌和肠炎沙门氏菌具有抗生素活性。它们在高达121oC的温度下20分钟以及在2.0至10.0的pH处理下仍保持活性。在冷冻条件下的储藏测试中，抗菌活性保持60天。产生的动力学揭示了在对数生长期的峰值活性为1600 AU / mL，发现的分子量约为3.0 kDa。具有抗微生物活性的产物的表征表明了它们的蛋白质性质，表现出主要代谢产物的典型动力学和与许多纯化肠毒素相似的分子量。

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