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首页> 外文期刊>Brazilian Journal of Microbiology >Bacteriocin like substance production by Carnobacterium piscicola in a continuous system with three culture broths. Study of antagonism against Listeria monocytogenes on vacuum packaged salmon
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Bacteriocin like substance production by Carnobacterium piscicola in a continuous system with three culture broths. Study of antagonism against Listeria monocytogenes on vacuum packaged salmon

机译：食肉杆菌（Carnobacterium piscicola）在具有三个培养液的连续系统中生产类似细菌素的物质。真空包装鲑鱼对单核细胞增生李斯特菌的拮抗作用研究

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Three culture media were studied for the bacteriocin like substance (BLS) production from Carnobacterium piscicola L 103 in a batch and continuous culture system. The efficacy of the antagonistic substance against Listeria monocytogenes was tested on vacuum packaged salmon. BLS was produced in a 1.0 L bioreactor by batch and continuous culture using D-MRS, mod. D-MRS and APT as nutrient broths. Salmon fillets were inoculated with BLS (200 AU mL-1 and 800 AU mL-1) and 8.0 x 101 cfu cm-2 of L. monocytogenes and stored at 4oC. The growth of L. monocytogenes was determined every 5 days during 15 days. After 12 h of batch culture the stationary growth phase of C. piscicola L 103 started, with a BLS activity of 800 AU mL-1 in D-MRS and mod. D-MRS broth, and of 400 AU mL-1 in APT broth. During continuous culture BLS activity increased to 6400 AU mL-1 in both types of MRS broths, while in APT the activity decreased to 50 AU mL-1, indicating a clear advantage of the first two culture media and also of the continuous culture system. BLS had a bacteriostatic effect on L. monocytogenes when inoculated on salmon, with counts of 6.0 x 103 cfu cm-2 after 15 days. No significant differences were found between the two BLS activities used. In the control without BLS, L. monocytogenes counts increased to 1.0 x 10(6) cfu cm-2 after 15 days of storage.
机译：在分批和连续培养系统中，研究了三种培养基用于从食肉弯曲杆菌L 103生产细菌素样物质（BLS）。在真空包装的鲑鱼上测试了拮抗物质对单核细胞增生性李斯特菌的功效。 BLS在1.0 L生物反应器中通过分批连续培养使用D-MRS，mod。 D-MRS和APT作为营养肉汤。用BLS（200 AU mL-1和800 AU mL-1）和8.0 x 101 cfu cm-2的单核细胞增生李斯特菌接种鲑鱼鱼片，并在4oC下保存。在15天内每5天测定一次单核细胞增生李斯特菌的生长。分批培养12 h后，C。piscicola L 103的固定生长期开始，在D-MRS和mod.s中的BLS活性为800 AU mL-1。 D-MRS肉汤，以及APT肉汤中的400 AU mL-1。在连续培养过程中，两种类型的MRS肉汤中BLS活性均增加至6400 AU mL-1，而在APT中，活性降低至50 AU mL-1，这表明前两种培养基以及连续培养系统均具有明显优势。 BLS接种鲑鱼后对单核细胞增生李斯特菌具有抑菌作用，15天后计数为6.0 x 103 cfu cm-2。在使用的两个BLS活动之间没有发现显着差异。在没有BLS的对照中，储存15天后，单核细胞增生李斯特菌计数增加到1.0 x 10（6）cfu cm-2。

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