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Endophytic fungi found in association with Bacopa monnieri as potential producers of industrial enzymes and antimicrobial bioactive compounds

机译:与Bacopa monnieri一起发现的内生真菌是工业酶和抗菌生物活性化合物的潜在生产者

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This study aimed to screen the endophytic fungal species of ethano-medicinal plant Bacopa monnieri (L.) Pennell for their ability to produce antimicrobial substances against Bacillus subtilis, Pseudomonas aeroginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Candida albicans. Endophytes were also screened for their ability to produce amylase, cellulase, protease and lipase to evaluate their ecological role within the host plant. Twenty-six endophytes were isolated and seventeen were identified. All the isolated endophytes exhibited amylolytic activity. Lipolytic, cellulolytic, proteolytic activity was shown by 98, 28 and 31% isolates, respectively. Similarly, all the endophytes (100%) exhibited significant antimicrobial activity against K. pneumonia, while seventeen endophytes (89.5%) were active against S. aureus. Fourteen endophytes (78.9%) showed significant antimicrobial activity against B. subtilis and C. albicans. Eleven (57.8%), nine (50%), four (21%) endophytes were active against S. typhimurium, E. coli and P. aeruginosa, respectively.
机译:这项研究的目的是筛选乙醇药用植物Bacopa monnieri(L.)Pennell的内生真菌物种,它们具有产生针对枯草芽孢杆菌,铜绿假单胞菌,鼠伤寒沙门氏菌,大肠杆菌,克雷伯菌肺炎,金黄色葡萄球菌和金黄色葡萄球菌的抗菌物质的能力。 。还筛选了内生菌产生淀粉酶,纤维素酶,蛋白酶和脂肪酶的能力,以评估其在宿主植物中的生态作用。分离出26种内生菌,鉴定出17种。所有分离的内生菌均表现出淀粉分解活性。分离物分别显示98%,28%和31%的脂解,纤维素分解和蛋白水解活性。同样,所有内生菌(100%)对肺炎克雷伯菌均表现出显着的抗菌活性,而十七种内生菌(89.5%)对金黄色葡萄球菌有活性。 14种内生菌(占78.9%)对枯草芽孢杆菌和白色念珠菌具有显着的抗菌活性。十一株(57.8%),九株(50%),四株(21%)内生菌分别对鼠伤寒沙门氏菌,大肠杆菌和铜绿假单胞菌具有活性。

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