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首页> 外文期刊>Bumi Lestari journal of environment >KONSERVASI ANGGREK ALAM INDONESIA Vanda tricolor Lindl. varietas suavis MELALUI KULTUR EMBRIO SECARA IN-VITRO
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KONSERVASI ANGGREK ALAM INDONESIA Vanda tricolor Lindl. varietas suavis MELALUI KULTUR EMBRIO SECARA IN-VITRO

机译：印度尼西亚自然兰花保护万达三色林德尔。 suavis品种通过体外胚胎培养

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Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for propagation of this species through in vitro culture could be nessecary for conservation purposes. High phenolic content of plant tissue is a serious problem for V. tricolor research in the laboratory, which inhibits germination and growth of the embryo. To overcome this problem, seeds were sown in a medium with the addition of tomato extract as an antioxidant. The aim of this research is to find the most suitable concentration of tomato extract for germination and growth of the embryo of V. tricolor form Bali and Merapi in order to obtained healthy seedlings for conservation purposes. Orchid pods (7 months after polination) of V. tricolor Bali and Merapi were used as plant material. The treatment consisted of 5 concentrations of tomato extract which is 0, 50 100, 150, 200, 250 g L-1. Observation was done by counting the number of protocorms for each stage of growth at 4 weeks after seed sowing. The study concluded that V. tricolor Bali is more responsive to the tomato extract compared with Merapi. Concentration of 150 gL-1 tomato extract gave the highest percentage of protocorms for Bali form, whereas Merapi form did not give significant differences either with or without tomato extract added in the culture medium.
机译：万代三色林德尔。变种suavis是一种印度尼西亚野生兰花，由于栖息地遭到破坏，现在在自然界中极为罕见。为了保护起见，开发适当的通过体外培养繁殖该物种的方法可能是必要的。植物组织中的高酚含量是实验室中三色拟青霉研究的一个严重问题，它抑制了胚的发芽和生长。为了克服这个问题，将种子播种在培养基中，加入番茄提取物作为抗氧化剂。这项研究的目的是找到最合适浓度的番茄提取物，用于巴厘和默拉皮三色弧菌的胚发芽和生长，从而获得用于保护目的的健康幼苗。 V. tricolor Bali和Merapi的兰花荚（授粉后7个月）被用作植物材料。处理包括5种浓度的番茄提取物，分别为0、50 100、150、200、250 g L-1。通过计数种子播种后4周每个生长阶段的原球茎数目来进行观察。该研究得出的结论是，与Merapi相比，V。tricolor Bali对番茄提取物反应更灵敏。浓度为150 gL-1的番茄提取物为Bali形式的原球茎百分比最高，而在培养基中添加或不添加番茄提取物的Merapi形式均无明显差异。

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