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Response of bacterioplankton community structure to an artificial gradient of ip/iCOsub2/sub in the Arctic Ocean

机译:北冰洋浮游植物群落结构对人工梯度 p CO 2 的响应

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In order to test the influences of ocean acidification on the ocean pelagicecosystem, so far the largest CO2 manipulation mesocosm study (EuropeanProject on Ocean Acidification, EPOCA) was performed in Kings Bay(Kongsfjorden), Spitsbergen. During a 30 day incubation, bacterial diversitywas investigated using DNA fingerprinting and clone library analysis ofbacterioplankton samples. Terminal restriction fragment length polymorphism(T-RFLP) analysis of the PCR amplicons of the 16S rRNA genes revealed thatgeneral bacterial diversity, taxonomic richness and community structure wereinfluenced by the variation of productivity during the time of incubation, butnot the degree of ocean acidification. A BIOENV analysis suggested a complexcontrol of bacterial community structure by various biological and chemicalenvironmental parameters. The maximum apparent diversity of bacterioplankton(i.e., the number of T-RFs) in high and low pCO2 treatments differedsignificantly. A negative relationship between the relative abundance ofBacteroidetes and pCO2 levels was observed for samples at theend of the experiment by the combination of T-RFLP and clone libraryanalysis. Our study suggests that ocean acidification affects the developmentof bacterial assemblages and potentially impacts the ecological function ofthe bacterioplankton in the marine ecosystem.
机译:为了测试海洋酸化对海洋中上层生态系统的影响,迄今为止,在斯匹次卑尔根的金斯湾(Kongsfjorden)进行了最大的CO 2 操纵中观研究(欧洲海洋酸化项目,EPOCA)。在30天的孵育过程中,使用DNA指纹图谱和浮游细菌样品的克隆文库分析研究了细菌多样性。对16S rRNA基因PCR扩增子的末端限制性片段长度多态性(T-RFLP)分析表明,在孵化过程中,生产力的变化影响了细菌的总体多样性,分类学丰富度和群落结构,但不影响海洋酸化的程度。 BIOENV分析表明,通过各种生物和化学环境参数可以复杂地控制细菌群落结构。高和低 p CO 2 处理中浮游细菌的最大表观多样性(即T-RFs数量)差异显着。在实验结束时,通过T-的组合,观察到样品中拟杆菌的相对丰度与CO 2 的相对丰度呈负相关。 RFLP和克隆文库分析。我们的研究表明,海洋酸化会影响细菌群落的发育,并可能影响海洋生态系统中浮游细菌的生态功能。

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