Management of DNA Information Bank Using Typifix Method for Microsatellite Analysis and Snp Assays in Order to Ensure Safety Foods By Animal Filiations and Traceability for Farm Animal Biodiversity Under the New Circumstances of the Eco-Economy Paradigm

A.T. BOGDAN; G. BREM; I. SEREGI; I. GROZA; Iudith IPATE; Monika GUTSCHER; G. F. TOBA; L. ZOLDAG; A. MORATI-AGOTS; Simona IVANA; L.D. DIACONESCU; STR?TEANU

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Management of DNA Information Bank Using Typifix Method for Microsatellite Analysis and Snp Assays in Order to Ensure Safety Foods By Animal Filiations and Traceability for Farm Animal Biodiversity Under the New Circumstances of the Eco-Economy Paradigm

机译：在生态经济范式的新情况下，使用Typifix方法进行DNA信息库管理以进行微卫星分析和Snp分析，以确保通过动物F变获得安全食品和农场动物生物多样性的可追溯性

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Molecular biology tests based on identification, amplification and characterization of nucleic acid revolutionized the pathology diagnostic, conservation of indigene animal genetic resources, gene assisted selection and food traceability from farm-to-fork and from fork-to-farm. The Deoxyribonucleic Acid (DNA) of each animal is different (with the exceptions of monozygotic twins and clones). Since the genome of each animal contains approximately three billion DNA units, the range for variation among the DNA sequences of animals is enormous, consequently DNA markers analysis allows assuring a traceability of 100% in the meat industry. A methodology using 17 ISAG (International Society for Animal Genetics) DNA microsatellite markers is proposed for meat traceability. The original methods - patented by Prof.Brem and his Agrobiogen team - that were used in Romania to reveal DNA polymorphism are described as their applicability in species identification and meat traceability. The objective it was analysis the DNA profile of pig with 10 microsatellite markers for meat identity control, of sheep for scrapie diagnostic and cattle for performants selection .

机译：基于核酸的鉴定，扩增和表征的分子生物学测试彻底改变了病理学诊断，靛蓝动物遗传资源的保存，基因辅助选择以及从农场到叉子以及从叉子到农场的食品可追溯性。每只动物的脱氧核糖核酸（DNA）不同（单卵双胞胎和克隆除外）。由于每只动物的基因组包含大约30亿个DNA单元，因此动物DNA序列之间的变异范围非常大，因此DNA标记分析可确保在肉类行业中实现100％的可追溯性。提出了一种使用17种ISAG（国际动物遗传学会）DNA微卫星标记的方法来进行肉类追溯。最初由Brem教授及其农杆菌生物学团队获得专利的方法被用于罗马尼亚揭示DNA多态性的方法被描述为在物种识别和肉类可追溯性方面的适用性。目的是分析具有10种微卫星标记的猪的DNA谱图，以控制肉的身份，对绵羊进行刮scrap诊断，对牛进行DNA谱图选择。

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《Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicin》 |2010年第1期|共9页
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A.T. BOGDAN; G. BREM; I. SEREGI; I. GROZA; Iudith IPATE; Monika GUTSCHER; G. F. TOBA; L. ZOLDAG; A. MORATI-AGOTS; Simona IVANA; L.D. DIACONESCU; STR?TEANU;
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中图分类动物医学（兽医学）;
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1. Development of improved analytical methods for use in animal health and in foodborne disease surveillance for source attribution. (Special Issue: Coordinating surveillance policies in animal health and food safety 'from farm to fork'.) [J] . Widen F., Leijon M., Engvall E. O., Revue Scientifique et Technique . 2013,第2期

机译：开发用于动物健康和食源性疾病监测的改进分析方法，以查明来源。（特刊：“从农场到餐桌”协调动物健康和食品安全的监督政策。）
2. Linking live animals and products: traceability. (Special Issue: Coordinating surveillance policies in animal health and food safety 'from farm to fork'.) [J] . Britt A. G., Bell C. M., Evers K., Revue Scientifique et Technique . 2013,第2期

机译：连接活体动物和产品：可追溯性。（特刊：“从农场到餐桌”协调动物健康和食品安全的监督政策。）
3. Trace analysis of three fungicides in animal origin foods with a modified QuEChERS method and liquid chromatography-tandem mass spectrometry [J] . Mu Zhaobin, Feng Xiaoxiao, Zhang Yun, Analytical and bioanalytical chemistry . 2016,第5期

机译：改进的QuEChERS方法和液相色谱-串联质谱法分析动物源食品中的三种杀菌剂
4. Using Typifix Method for Microsatellite Analysis and SNP Assays with using the Genetic Biodiversity Indicator for Identification in Ewes of Romania Genotypes Valuable Resistant in Scrapie [C] . IUDITH IPATE, ALEXANDRU T. BOGDAN, BREM G, Advances in biology, bioengineering and environment . 2010

机译：使用Typifix方法进行微卫星分析和SNP分析，并使用遗传生物多样性指标鉴定罗马尼亚基因型对稀有抗性的母羊
5. A Comparative Analysis of Consumer Attitudes Towards Food Safety, Animal Testing and Traceability in the Meat Industry: Japan and Canada [D] . Aubeeluck, Ashwina 2010

机译：消费者对肉类行业食品安全，动物测试和可追溯性态度的比较分析：日本和加拿大

Management of DNA Information Bank Using Typifix Method for Microsatellite Analysis and Snp Assays in Order to Ensure Safety Foods By Animal Filiations and Traceability for Farm Animal Biodiversity Under the New Circumstances of the Eco-Economy Paradigm

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