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首页> 外文期刊>Cancer and Clinical Oncology >The Effect of Human Papillomavirus E6 Oncogene on the Radiosensitivity of Non-Oropharyngeal Cancer Cells
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The Effect of Human Papillomavirus E6 Oncogene on the Radiosensitivity of Non-Oropharyngeal Cancer Cells

机译：人乳头瘤病毒E6癌基因对非口咽癌细胞放射敏感性的影响

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Background:We have previously shown that stable transfection of the human papillomavirus (HPV) E6*I oncogene can sensitize two HPV negative oropharyngeal cancer (OSCC) cell lines to radiation. In the current study, we extended our work on OSCC to determine whether the HPV E6 oncogene can enhance the radiosensitivity of non-OSCC cell lines.Methods:Three non-OSCC cell lines (melanoma, colorectal adenocarcinoma and large cell lung cancer) were stably transfection with the HPV E6 oncogene (E6 total, E6*I and E6*II) and treated with different doses of radiation. Clonogenic assays were used to measure the radiation survival.Results:Following transfection, there was a reduction in the survival of the melanoma cell line after 2 Gy (SF2) from 0.401 (untransfected) to 0.219 (Melanoma-E6 total). This reduction was not evident at higher doses of radiation. There was no significant change in the SF2 of melanoma-E6*I (0.303) and melanoma-E6*II (0.414). The SF2 colorectal adenocarcinoma and large cell lung cancer cell lines did not change significantly after transfection.Conclusions: The radiosensitizing effect of HPV E6 oncogene is cell line specific. We found no clear evidence of a radiosensitising effect of E6 in these three non-OSCC cancer cell lines.
机译：背景：我们之前已经证明，人乳头瘤病毒（HPV）E6 * I癌基因的稳定转染可以使两种HPV阴性口咽癌（OSCC）细胞系对放射敏感。在本研究中，我们扩展了对OSCC的研究，以确定HPV E6癌基因是否可以增强非OSCC细胞系的放射敏感性。方法：三种非OSCC细胞系（黑色素瘤，结直肠腺癌和大细胞肺癌）稳定用HPV E6癌基因（共E6，E6 * I和E6 * II）转染，并用不同剂量的放射线处理。结果：转染后，2 Gy（SF2）后黑色素瘤细胞系的存活率从0.401（未转染）降低到0.219（黑色素E6总数）。在较高剂量的辐射下这种减少并不明显。黑色素瘤-E6 * I（0.303）和黑色素瘤-E6 * II（0.414）的SF2无明显变化。转染后SF2大肠腺癌和大细胞肺癌细胞株无明显变化。结论：HPV E6癌基因的放射增敏作用是细胞株特异的。我们没有发现在这三种非OSCC癌细胞系中E6的放射增敏作用的明确证据。

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