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首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >MELITTIN – INDUCED CHANGES IN LIPID BILAYERS: A MOLECULAR ACOUSTIC STUDY
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MELITTIN – INDUCED CHANGES IN LIPID BILAYERS: A MOLECULAR ACOUSTIC STUDY

机译：蜂毒肽-脂质双脂引起的变化：分子声学研究

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摘要

The effect of melittin at mole fractions of up to 3.75mol%, on the dynamic and mechanical properties of 1,2-dipalmitoyl- sn-glycero-3-phosphatidylcholine (DPPC) was investigated using ultrasound velocimetry and densitometry. The isothermal compressibility and volume fluctuations of DPPC – melittin bilayer membrane in their different transition phases were determined. At the melting temperature, T m , a general increase in velocity number [u] as compared to the pure lipid was noticed. However, at 3.75 mol%, melittin displays the broadest peak and lowest value of [u] compared to the other melittin concentrations, which could be linked to the lytic property of melittin at high peptide concentrations. The melittin concentration effects on the partial specific volume, ν o and the adiabatic compressibility coefficient of the lipid, β Slipid of the DPPC bilayer reveals a slight shift of the main transition to lower temperature and a continuous decrease in ν o with concentration increase above the T m (except between 1 and 1.25%), as well as a decrease in β Slipid with decrease in melittin concentration. Again, the isothermal compressibility peak at the main transition drops drastically upon addition of melittin at concentrations as low as 1 mol% and then increases with increase in melittin concentration. At all melittin concentrations, β Tlipid is greater than β Slipid in the gel-fluid region by ～ 20%. The maximum value of the relative volume fluctuations of 12 % is reached for DPPC at the main transition, and is strongly dampened upon addition of melittin. Only a slight decrease in the calculated relative volume fluctuations with melittin concentration is observed between concentrations 0 and 1.0 mol%.
机译：使用超声测速和光密度法研究了蜂毒肽在高达3.75mol％的摩尔分数下对1,2-二棕榈酰-sn-甘油-3-磷脂酰胆碱（DPPC）的动力学和机械性能的影响。确定了DPPC-蜂毒肽双层膜在不同过渡阶段的等温压缩性和体积波动。在熔化温度T m处，与纯脂质相比，速度数[u]普遍增加。然而，与其他蜂肽浓度相比，蜂毒素在3.75 mol％时显示出最宽的峰和最低的[u]值，这可能与蜂肽在高肽浓度下的裂解特性有关。蜂毒肽浓度对脂质的部分比容νo和绝热可压缩系数的影响，DPPC双层的β脂质显示出向较低温度的主转变略有偏移，并且随着浓度的增加，νo连续降低。 T m（1-1.25％除外），以及随着蜂毒肽浓度的降低，β血脂降低。同样，在加入低至1 mol％浓度的蜂毒素后，主转变处的等温可压缩性峰急剧下降，然后随着蜂毒素浓度的增加而增加。在所有蜂毒肽浓度下，凝胶流体区域中的β脂质大于β脂质约20％。 DPPC在主要过渡段达到相对体积波动的最大值12％，并在加入蜂毒肽后被强烈抑制。在浓度为0到1.0摩尔％之间，随蜂毒蛋白的浓度计算的相对体积波动仅略有减少。

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