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首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >ENZYMATIC SURFACE HYDROLYSIS OF POLY(ETHYLENETEREPHTHALATE) BY LIPASE ENZYME AND ITS PRODUCTION
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ENZYMATIC SURFACE HYDROLYSIS OF POLY(ETHYLENETEREPHTHALATE) BY LIPASE ENZYME AND ITS PRODUCTION

机译：脂肪酶酶解表面聚合的对苯二甲酸乙二醇酯及其生产

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摘要

In this study, hydrolysis of the surface of poly(ethyleneterephthalate) (PET) fabric by Bacillus isolate 5W and 6C lipases was confirmed by improved hydrophilic property which tested by wetability and staining with basic dyes, scanning electron microscope, and FTIR measurements. Lipase treated PET fabrics showed good physical and mechanical properties. It was observed that the retained strength of lipase treated PET was 96-98% and pilling and static charges were reduced. Bacillus isolate 5W was chosen for economic production of PET surface modifying lipase enzyme in 14 agro-industrial by-products. The most suitable substrate for the highest lipase production was cotton seed meal at 6% and initial pH 7.5 (90U/ml). Effect of volume of the medium in the flask, inoculums size and incubation period were investigated and 25 ml of the medium, 25 x 10 6 CFU/ml, and 24h, respectively were the optimal. Addition of peptone to cotton seed meal medium enhanced the lipase production 22.5% (130.7 U/ml). The results of this article focuses on production of lipase enzyme that hydrolyze PET under feasible and economic conditions for replacing harsh chemical processes currently used for hydrophilization.
机译：在这项研究中，芽孢杆菌分离的5W和6C脂肪酶对聚对苯二甲酸乙二醇酯（PET）织物表面的水解通过改善的亲水性得到了证实，该亲水性通过润湿性和碱性染料染色，扫描电子显微镜和FTIR测量进行了测试。经脂肪酶处理的PET织物显示出良好的物理和机械性能。观察到，经脂肪酶处理的PET的保留强度为96-98％，起球和静电荷降低。选择了芽孢杆菌分离株5W来经济生产14种农用工业副产物中的PET表面修饰脂肪酶。最适合产生最高脂肪酶的底物是6％的棉籽粕，初始pH 7.5（90U / ml）。研究了烧瓶中培养基体积，接种量和孵育时间的影响，最佳培养基为25 ml，25 x 10 6 CFU / ml和24h。将蛋白one添加到棉籽粉培养基中可使脂肪酶的产量提高22.5％（130.7 U / ml）。本文的结果集中在脂肪酶的生产上，该酶在可行和经济的条件下水解PET，以替代目前用于亲水化的苛刻化学过程。

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