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首页> 外文期刊>Cancer Cell International >Colocalization of somatostatin receptors and epidermal growth factor receptors in breast cancer cells
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Colocalization of somatostatin receptors and epidermal growth factor receptors in breast cancer cells

机译：生长抑素受体和表皮生长因子受体在乳腺癌细胞中的共定位

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Background Somatostatin receptor (SSTR) expression is positively correlated with tumor size and inversely correlated with epidermal growth factor receptor (ErbB) levels and tumor differentiation. In the present study, we compared SSTR1-5 and ErbB1-4 mRNA and protein expression in two breast cancer cell lines: MCF-7 (ER+) and MDA-MB-231 (ERα-). Results All five SSTRs and four ErbBs were variably expressed as both cell surface and cytoplasmic proteins. In both cell lines, SSTR4 and SSTR1 were highly expressed, followed by SSTR2 and SSTR5 with SSTR3 being the least expressed subtype, at the protein level. ErbBs were variably expressed with ErbB1 as the predominant subtype in both cell lines. ErbB1 is followed by ErbB3, ErbB2 and ErbB4 in MCF-7 at both the protein and mRNA levels. In MDA-MB-231 cells, ErbB1 is followed by ErbB2, ErbB4 and ErbB3. Our results indicate significant correlations at the level of mRNA and protein expression in a cell and receptor-specific manner. Using indirect immunofluorescence, we found that, in MCF-7 cells, SSTR5 was the most prominent subtype coexpressed with ErbBs followed by SSTR3, SSTR4, SSTR1 and SSTR2, respectively. In MDA-MB-231 cells, SSTR1 colocalized strongly with ErbBs followed by SSTR5, SSTR4, SSTR3 and SSTR2. ErbBs displayed higher levels of colocalization amongst themselves in MCF-7 cells than in MDA-MB-231 cells. Conclusion These findings may explain the poor response to endocrine therapy in ER-cancer. Differential distribution of SSTR subtypes with ErbBs in breast cancer cells in a receptor-specific manner may be considered as a novel diagnosis for breast tumors.
机译：背景生长抑素受体（SSTR）的表达与肿瘤的大小呈正相关，与表皮生长因子受体（ErbB）的水平和肿瘤的分化呈负相关。在本研究中，我们比较了两种乳腺癌细胞系MCF-7（ER +）和MDA-MB-231（ERα-）中SSTR1-5和ErbB1-4 mRNA和蛋白的表达。结果所有5个SSTR和4个ErbB均以细胞表面和细胞质蛋白的形式可变表达。在两种细胞系中，在蛋白质水平上，SSTR4和SSTR1均高表达，其次是SSTR2和SSTR5，其中SSTR3是表达最少的亚型。 ErbBs在两种细胞系中均以ErbB1作为主要亚型而可变表达。在蛋白质和mRNA水平上，MCF-7中的ErbB1之后是ErbB3，ErbB2和ErbB4。在MDA-MB-231单元中，ErbB1之后是ErbB2，ErbB4和ErbB3。我们的结果表明以细胞和受体特异性方式在mRNA和蛋白质表达水平上存在显着相关性。使用间接免疫荧光，我们发现在MCF-7细胞中，SSTR5是与ErbBs共表达的最突出的亚型，其次分别是SSTR3，SSTR4，SSTR1和SSTR2。在MDA-MB-231细胞中，SSTR1与ErbBs强烈共定位，随后是SSTR5，SSTR4，SSTR3和SSTR2。与在MDA-MB-231细胞中相比，ErbBs在MCF-7细胞中显示出更高的共定位水平。结论这些发现可能解释了ER-癌症对内分泌治疗的不良反应。具有ErbBs的SSTR亚型在乳腺癌细胞中以受体特异性方式的差异分布可被认为是乳腺癌的一种新颖诊断方法。

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