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首页> 外文期刊>Behavioral and Brain Functions >Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression
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Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression

机译：骨髓源间充质干细胞（BMSCs）的移植改善大鼠脑缺血诱导的与TNF-α表达相关的肺损伤

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Background Bone marrow mesenchymal stem cell (BMSCs)-based therapy seems to be a promising treatment for acute lung injury, but the therapeutic effects of BMSCs transplantation on acute lung injury induced by brain ischemia and the mechanisms have not been totally elucidated. This study explores the effects of transplantation of BMSCs on acute lung injury induced by focal cerebral ischemia and investigates the underlying mechanism. Methods Acute lung injury model was induced by middle cerebral artery occlusion (MCAO). BMSCs (with concentration of 1?×?106/ml) were transplanted into host through tail vein 1?day after MCAO. Then, the survival, proliferation and migration of BMSCs in lung were observed at 4?days after transplantation, and histology observation and lung function were assessed for 7?days. Meanwhile, in situ hybridization (ISH), qRT-PCR and western blotting were employed to detect the expression of TNF-α in lung. Results Neurobehavioral deficits and acute lung injury could be seen in brain ischemia rats. Implanted BMSCs could survive in the lung, and relieve pulmonary edema, improve lung function, as well as down regulate TNF-α expression. Conclusions The grafted BMSCs can survive and migrate widespread in lung and ameliorate lung injury induced by focal cerebral ischemia in the MCAO rat models. The underlying molecular mechanism, at least partially, is related to the suppression of TNF-α.
机译：背景技术基于骨髓间充质干细胞（BMSCs）的治疗似乎是治疗急性肺损伤的一种有前途的治疗方法，但是BMSCs移植对脑缺血引起的急性肺损伤的治疗作用及其机制尚未完全阐明。本研究探讨了骨髓间充质干细胞移植对局灶性脑缺血所致急性肺损伤的作用，并探讨了其潜在机制。方法采用大脑中动脉闭塞（MCAO）诱导急性肺损伤模型。在MCAO后1天，通过尾静脉将BMSCs（浓度为1？×？10 6 / ml）移植到宿主体内。然后，在移植后4天观察肺中BMSC的存活，增殖和迁移，并在7天时评估组织学观察和肺功能。同时，采用原位杂交（ISH），qRT-PCR和western blotting检测肺组织TNF-α的表达。结果脑缺血大鼠可见神经行为缺陷和急性肺损伤。植入的骨髓间充质干细胞可以在肺中存活，并减轻肺水肿，改善肺功能并下调TNF-α的表达。结论在MCAO大鼠模型中，移植的BMSCs可以在肺中生存和迁移，减轻局灶性脑缺血所致的肺损伤。潜在的分子机制至少部分与抑制TNF-α有关。

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