...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell Division >lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2
【24h】

lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2

机译：lemmingA编码果蝇中APC / C的Apc11亚基，与E2-C型泛素结合酶Vihar和Morula / Apc2形成三元复合物

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Ubiquitin-dependent protein degradation is a critical step in key cell cycle events, such as metaphase-anaphase transition and mitotic exit. The anaphase promoting complex/cyclosome (APC/C) plays a pivotal role in these transitions by recognizing and marking regulatory proteins for proteasomal degradation. Its overall structure and function has been elucidated mostly in yeasts and mammalian cell lines. The APC/C is, however, a multisubunit assembly with at least 13 subunits and their function and interaction within the complex is still relatively uncharacterized, particularly in metazoan systems. Here, lemming (lmg) mutants were used to study the APC/C subunit, Apc11, and its interaction partners in Drosophila melanogaster. Results The lmg gene was initially identified through a pharate adult lethal P element insertion mutation expressing developmental abnormalities and widespread apoptosis in larval imaginal discs and pupal abdominal histoblasts. Larval neuroblasts were observed to arrest mitosis in a metaphase-like state with highly condensed, scattered chromosomes and frequent polyploidy. These neuroblasts contain high levels of both cyclin A and cyclin B. The lmg gene was cloned by virtue of the lmg03424 P element insertion which is located in the 5' untranslated region. The lemming locus is transcribed to give a 2.0 kb mRNA that contains two ORFs, lmgA and lmgB. The lmgA ORF codes for a putative protein with more than 80% sequence homology to the APC11 subunit of the human APC/C. The 85 amino acid protein also contains a RING-finger motif characteristic of known APC11 subunits. The lmgA ORF alone was sufficient to rescue the lethal and mitotic phenotypes of the lmg138 null allele and to complement the temperature sensitive lethal phenotype of the APC11-myc9 budding yeast mutant. The LmgA protein interacts with Mr/Apc2, and they together form a binding site for Vihar, the E2-C type ubiquitin conjugating enzyme. Despite being conserved among Drosophila species, the LmgB protein is not required for viability or fertility. Conclusions Our work provides insight into the subunit structure of the Drosophila APC/C with implications for its function. Based on the presented data, we suggest that the Lmg/Apc11 subunit recruits the E2-C type ubiquitin conjugating enzyme, Vihar, to the APC/C together with Mr/Apc2 by forming a ternary complex.
机译：背景泛素依赖性蛋白质降解是关键细胞周期事件（如中期-后期转变和有丝分裂退出）中的关键步骤。后期促进复合物/环体（APC / C）通过识别和标记蛋白酶体降解的调节蛋白在这些过渡过程中发挥关键作用。大多数酵母和哺乳动物细胞系已经阐明了其总体结构和功能。然而，APC / C是具有至少13个亚基的多亚基组件，并且它们在复合物中的功能和相互作用仍然相对较不表征，特别是在后生动物系统中。在这里，lemming（lmg）突变体用于研究果蝇中的APC / C亚基Apc11及其相互作用伙伴。结果最初通过一个成年的致命性P元素插入突变鉴定了lmg基因，该突变在幼虫假想盘和p腹部成纤维细胞中表达发育异常和广泛的细胞凋亡。观察到幼虫成神经细胞以高度浓缩，分散的染色体和频繁的多倍性在中期状态下阻止有丝分裂。这些成神经细胞含有高水平的细胞周期蛋白A和细胞周期蛋白B。lmg基因是通过位于5'非翻译区的lmg 03424 P元件插入而克隆的。旅鼠基因座被转录以产生一个包含两个ORF（1mgA和1mgB）的2.0 kb mRNA。 lmgA ORF编码与人APC / C的APC11亚基具有80％以上序列同源性的推定蛋白质。该85个氨基酸的蛋白质还包含已知APC11亚基特征的RING-手指基序。单独使用lmgA ORF足以拯救lmg 138 无效等位基因的致死和有丝分裂表型，并补充APC11-myc9出芽酵母突变体对温度敏感的致死表型。 LmgA蛋白与Mr / Apc2相互作用，它们共同形成E2-C型泛素结合酶Vihar的结合位点。尽管在果蝇物种中是保守的，但LmgB蛋白并不是生存力或繁殖力所必需的。结论我们的工作深入了解了果蝇APC / C的亚基结构及其功能。根据提出的数据，我们建议Lmg / Apc11亚基通过形成三元复合物将E2-C型泛素结合酶Vihar与Mr / Apc2一起募集到APC / C。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号