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首页> 外文期刊>Cellular Oncology: Analytical Cellular Pathology >Flow Cytometric Bivariate Analysis of DNA and Cytokeratin in Colorectal Cancer
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Flow Cytometric Bivariate Analysis of DNA and Cytokeratin in Colorectal Cancer

机译：大肠癌中DNA和细胞角蛋白的流式细胞术双变量分析

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Different opinions about flow cytometric estimates of DNA aneuploidy and/or S‐phase fraction (SPF) as supplementary prognostic markers in colorectal cancer are to some degree associated with methodology. Using univariate DNA analysis, we have previously investigated the DNA ploidy in colorectal cancer, its heterogeneity within and between tumors and its relation to survival. To improve detection of DNA aneuploid subpopulations and particularly estimation of their SPF's we investigated a method for bivariate DNA/cytokeratin analysis on fine‐needle aspirates of 728 frozen biopsies from 157 colorectal tumors. Unfixed aspirates were stained with propidium iodide and FITC‐conjugated anti‐cytokeratin antibody in a saponin‐buffer. A significant association between SPF and debris was observed. There were no substantial difference in DNA ploidy patterns between univariate and bivariate measurements (concordance was 92–95%). No new DNA aneuploid subpopulations were detected in cytokeratin‐gated compared to ungated or univariate histograms. Debris‐adjusted SPF's of cytokeratin‐gated histograms were significantly higher than of ungated histograms, also for subpopulations with DI>1.4 (p < 0.0001). There was no significantassociation between SPF and survival.
机译：关于DNA非整倍性和/或S期分数（SPF）作为大肠癌辅助预后标志物的流式细胞术估计的不同观点在某种程度上与方法学相关。使用单变量DNA分析，我们以前已经研究了结直肠癌中的DNA倍性，其在肿瘤内和肿瘤之间的异质性以及与生存的关系。为了改善对DNA非整倍体亚群的检测，尤其是对其SPF的估计，我们研究了对来自157个大肠肿瘤的728例冷冻活检的细针抽吸物进行双变量DNA /细胞角蛋白分析的方法。未固定的吸出液用碘化丙锭和FITC偶联的抗细胞角蛋白抗体在皂素缓冲液中染色。观察到SPF与碎片之间的显着关联。单变量和双变量测量之间的DNA倍性模式没有实质性差异（一致性为92–95％）。与无门槛或单变量直方图相比，在细胞角蛋白门控中未检测到新的DNA非整倍体亚群。细胞角蛋白门控直方图的碎片调整后SPF显着高于非门控直方图，对于DI> 1.4的亚群也是如此（p <0.0001）。 SPF与生存率之间无显着关联。

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